三氧化二砷对血管内皮细胞增殖和周期的影响

目的:观察As2O3对血管内皮细胞增殖、凋亡和细胞周期的干扰,探讨As2O3对血管内皮细胞生长的直接影响。方法:血管内皮细胞EVC-304体外培养,As2O3干预。采用MTT测定细胞活性,流式细胞仪测定细胞周期、细胞凋亡。结果:ECV-304细胞经过As2O3干预,其存活率明显降低,并且呈剂量依赖性。As2O3干预后,进入S期的细胞减少,细胞周期被阻在G1期,细胞可能在G1期进入凋亡。As2O3诱导早期凋亡是对照组的2.88～5.1倍,并且呈剂量依赖性;其晚期凋亡是对照组的1.17～1.67倍。结论:As2O3可直接干扰血管内皮细胞的细胞周期,阻滞细胞在G1期,并诱导细胞凋亡,进而抑制血管内皮细胞的增殖。

Effects of arsenic trioxide on proliferation and cell cycle of vascular endothelial cells

Objective:To study the effecs on effect of arsenic trioxide on proliferation and cell cycle of vascular endothelial cells.Methods:ECV304 cells were treated with As2O3.Cell viability of ECV304 cells was measured by MTT assay.Cell cycle was analyzed by using flow cytometry with PI staining;apoptosis was estimated by flow cytometry by Annexin V-FITC conjugated with PI staining.Results:FCM assays showed that the percent of the cells treated with As2O3 were higher in sub-G1,lower in sub-S and lower in G2/G1 than that of the control,and 14.84% and 18.9% of the cells treated with As2O3 at 2.5μmol/L and 5μmol/L respectively were in apoptosis.Early apoptosis in As2O3 treated groups were 2.88~5.1 times higher than that of the control,late apoptosis in As2O3 treated groups were 1.17~1.67 times higher than that of the control.Conclusions:As2O3 may directly inhibit proliferation of vascular endothelial cells and induce apoptosis.