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| 耐甲氧西林金黄色葡萄球菌femA调控基因的研究 | |
| 引用本文： | 李晓芳,范昕建,冯萍,唐红,张国庆. 耐甲氧西林金黄色葡萄球菌femA调控基因的研究[J]. 西部医学, 2010, 22(11): 1983-1985 |
| 作者姓名： | 李晓芳 范昕建 冯萍 唐红 张国庆 |
| 作者单位： | 1. 成都市第一人民医院消化科,成都市中西医结合医院,四川,成都,610041 2. 四川大学华西医院感染性疾病中心,生物治疗国家重点实验室,四川,成都,610041 3. 双流县新兴镇卫生院,四川,双流,610213 |
| 基金项目： | 国家自然科学基金赞助项目，国际合作CMB费助项目 |
| 摘 要： | 目的探讨耐甲氧西林金黄色葡萄球菌的辅助基因femA是否存在调控基因,以进一步揭示其耐药机制。方法用最低抑菌浓度法（MIC）筛选对苯唑西林高耐株、低耐株、敏感株;用头孢色芬纸片法（Nitrocephin）筛选出不产β-内酰胺酶菌株;用PCR筛选出含mecA的菌株。将含mecA不产酶的高耐株、低耐株、敏感株纳入进一步研究中;PCR扩增femA及femA5？上游基因;提取细菌总蛋白;生物素标记DNA探针;滞留电泳检测调控基因。结果滞留电泳检测对苯唑西林高度耐药不产β-内酰胺酶含mecA基因菌株均出现滞留条带。结论耐甲氧西林金黄色葡萄球菌的辅助基因femA有调控基因存在。 |
| 关 键 词： | 耐甲氧西林金黄色葡萄球菌 滞留电泳 femA 调控基因 |
Study on regulating gene femA in methicillin-resistant Staphytococcus aureus(MRSA) |
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| Affiliation: | LI Xiao-fang,FAN Xin-jian,FENG Ping,et al(1.Department of Digestive System,The First People＇s Hospital of Chengdu,China;2.Department of Infectious Diseases,West China Hospital,Sichuan University,Chengdu 610041,China) |
| Abstract: | Objective To research the regulating gene of femA in methicillin-resistant Staphytococcus aureus.Methods Strains of Staphytococcus aureus were distinguished as high-resistant,low-resistant and sensitive to oxacillin with MIC.Strans of non-producedβ-lactamase were selected with Nitrocephin.Strains with mecA were selected with PCR.The regulating gene was detected with electrophoretic Mobility Shift Assay（EMSA）.Results All MRSA of no express β-lactamase had mecA gene Conclusion The regulating gene of femA probably lies in the 250 bp upstream sequence in MRSA. |
| Keywords: | femA |
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