510nm激光诱导血管平滑肌细胞凋亡的初步研究

为了探讨用激光防治血管成形术后再狭窄的可能性，以５１０ｎｍ激光照射体外培养的血管平滑肌细胞，以ＤＮＡ片断末端标记法观察细胞凋亡现象。结果显示，５０和７５Ｊ／ｃｍ２照射后４小时ＳＭＣ出现凋亡现象，２４小时凋亡最为显著，凋亡率分别为９．４％和６．１％。表明激光直接照射能引起平滑肌细胞凋亡。这可能有益于减轻血管内膜的增厚程度

Apoptosis of Vascular Smooth Muscle Cells Induced by 510 nm Laser Irradiation

We investigated the effect of laser irradiation to induce apoptosis of vascular smooth muscle cells(SMC). Rabbit SMC, isolated from the thoracic arota, were cultured in DMEM containing 10% fetal calf serum. SMC from passages 5 and 6 were irradiated by 510 nm copper vapor laser with the power densities of 25, 50, 75, 100, 200 mW/cm 2 and the energy densities of 25, 50, 75, 100, 200 J/cm 2. Apoptosis of SMC were identified by specific labeling of nuclear DNA fragment (TUNEL) at 4, 12, 24, 48 and 72 hours after irradiation and the necrotic cells were determined by the trypan blue dye exclusion test. It was found that SMC began to undergo apoptosis at 4 hours after irradiation by 50 and 75 J/cm 2 and the number of apoptotic cells reached a maximum at 24 hours. This result indicated that 510 nm copper vapor laser irradiation may induce apoptosis of SMC and it should attract attention as a possible approach to reduce coronary restenosis.