受体介导的c-myc反义核酸提高肝癌细胞对不同化疗药物的敏感性

目的: 探讨半乳糖(Galactose,Gal)-聚乙烯亚胺(Polyethyleneimine,PEI)-c-myc反义核酸(Antisense-Oligonucleotides,ASODN)复合物联合化疗药物三氧化二砷(Arsenic Trioxide As₂O₃),5-氟脲嘧啶(5-Fluorouracil,5-FU),表阿霉素(Pharmorbincin,PHA)对人肝癌Bel-7402细胞增殖的抑制作用。 方法: 选用不同浓度的As₂O₃、5-FU、PHA单独使用、联合c-myc ASODN、联合Gal-PEI-ASODN,作用于Bel-7402细胞,采用WST-8法检测细胞增殖的抑制率,并计算药物作用的IC₅₀。 结果: 不同浓度的化疗药物(As₂O₃,5-FU,PHA)联合0.25μmol/LGal-PEI-ASODN均明显降低了化疗药物的IC₅₀,提高了Bel-7402细胞对化疗药物的敏感性(分别提高药效3.38,1.58,2.05倍)。 结论: 半乳糖受体介导的c-myc反义核酸能提高人肝癌Bel-7402细胞对As₂O₃,5-FU,PHA的敏感性,与As2O3联合作用效果最好。

Receptor Mediated C-myc ASODN Enhancing Chemotherapeutic Sensitivities on Bel-7402 Cell Line

Objective : This experiment used galactose (Gal)-polyethyleneimine (PEI) as a vector,delivered c-m yc antisense oligodeoxynucleotides (ASODN) to human hepatocellular carcinoma Bel-7402 cells in order to investigate if Gal -PEI -ASODN complex can enhance drug sensitivity on Bel-7402 cell lines with arsenic trioxide (Arsenic Trioxide, As₂O₃), 5 -fluorouracil (5 - FU) and Pharmorbincin (PHA). Methods : Bel-7402 cells incubated with different concentrations of As₂O₃, 5-FU,PHA alone or combining with c-myc ASODN or Gal-PEI-ASODN. The inhibitory rate of cell proliferation was tested using a cell counting kit (WST - 8 method) and the IC 50 was calculated. Results : Different chemotherapeutic drugs (As₂O₃; 5-FU, PHA) combined with Gal -PEI - ASODN (0.25μmol/L) reduced the IC 50 on Bel-7402 cells and chemotherapeutic drug's sensitivity was enhanced 3.58, 1.58 and 2.05 times comparing with pure chemotherapeutic drug group respectively. Conclusions : Gal -PEI-A -SODN can enhance chemotherapeutic drug's sensitivity on Bel - 7402 cells of As₂O₃, 5 -FU, PHA. The best combined effect is tested in As₂O₃ combining with Gal-PEI-ASODN group.