Ooplasmic injection of elongating spermatids for the treatment of non- obstructive azoospermia |
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| Authors: | Sofikitis NV; Yamamoto Y; Miyagawa I; Mekras G; Mio Y; Toda T; Antypas S; Kawamura H; Kanakas N; Antoniou N; Loutradis D; Mantzavinos T; Kalianidis K; Agapitos E |
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| Institution: | Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan. |
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| Abstract: | We applied the technique of ooplasmic elongating spermatid injection to the
treatment of non-obstructive azoospermia. Mature oocytes were injected with
elongating spermatids isolated from testicular biopsy material obtained
from 13 non-obstructed azoospermic men. Seventy-three oocytes were
successfully injected with elongating spermatids and were then cultured for
36 h. At 13 h post-injection 68 oocytes were found to be activated and 52
of them were fertilized. Forty-one 2- to 4-cell stage embryos developed
from normally fertilized oocytes were transferred. At least two embryos
were transferred to each female partner. Two pregnancies were achieved.
Elongating spermatid injection may have a role in the treatment of
non-obstructive azoospermia.
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