| 益髓灵氯仿提取物诱导HL—60细胞凋亡的初步研究 |
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| 引用本文： | 陈信义,邵德彬.益髓灵氯仿提取物诱导HL—60细胞凋亡的初步研究[J].中医杂志,1999,40(4):235-236. |
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| 作者姓名： | 陈信义 邵德彬 |
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| 作者单位： | 北京中医药大学东直门医院，中国中医研究院，北京中医药大学东直门医院，北京中医药大学东直门医院，中国中医研究院，中国中医研究院，中国中医研究院 北京东城区海运仓 100700，北京东城区海运仓 100700，北京东城区海运仓 100700 |
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| 基金项目： | 国家自然科学基金课题部分内容(批准号:3947863) |
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| 摘 要： | 本项研究重点进行了益髓灵氯仿提取物、含氯仿提取物的兔血清、含氯仿提取物的兔肝匀浆、足叶乙甙、正常兔血清五组诱导HL—60细胞凋亡效应的实验研究。结果显示,短期液体培养后,取培养的HL—60细胞,经光学显微镜观察与流式细胞仪分析,前四组均见明显的HL—60细胞凋亡现象。其中,含益髓灵氯仿提取物的兔肝匀浆作用最强,与阳性对照药足叶乙甙相似,兔血清作用次之,益髓灵氯仿提取物作用较弱,正常兔血清无明显的诱导HL—60细胞凋亡作用。对于其诱导凋亡机理尚正在研究之中。
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| 关 键 词： | 白血病 益髓灵 氯仿提取物 HL－60 中医药疗法 |
Preliminary Study on HL -60 Cellular Wither Induced by Chloroform Extract of Yisuiling |
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| Chen Xinyi,Shao Debin,Zhao Liye,et al..Preliminary Study on HL -60 Cellular Wither Induced by Chloroform Extract of Yisuiling[J].Journal of Traditional Chinese Medicine,1999,40(4):235-236. |
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| Authors: | Chen Xinyi Shao Debin Zhao Liye |
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| Institution: | Chen Xinyi,Shao Debin,Zhao Liye,et al.
Dongzhimen Hospital,Beijing University of TCM,Beijing 100700 ' |
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| Abstract: | The experiment was conducted on the actions of 5 kinds of reagents on HL - 60 cellular wither, viz. Yisuiling chloroform extract, rabbit serum chloroform extract, rabbit liver paste chloroform extract, Etoposide, and normal rabbit serum. Results revealed that after fluid incubation for a short period, the HL-60 cultured cells showed no wither in the former 4 groups under microscope and flowing cytometer. Among them, action was strongest in rabbit liver paste of Yisuiling extract group, similar to the positive control of Etoposide. Followed by rabbit serum, and the action was weak in Yisuiling chloroform extract. There was no obvious action of induced wither by normal rabbit serum. The mechanism of induced wither is still under investigation. |
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| Keywords: | Yisuiling cell culture cell wither dephagocytosis/drug action magakaryocyte/drug action @ Yisuiling chloroform extract |
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