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白木香核型与Giemsa C-带带型研究
引用本文:申彦晶,赵树进.白木香核型与Giemsa C-带带型研究[J].中药材,2007,30(7):762-765.
作者姓名:申彦晶  赵树进
作者单位:1. 华南理工大学生物科学与工程学院,广东广州,510641
2. 华南理工大学生物科学与工程学院,广东广州,510641;广州军区广州总医院药学部,广东广州,510010
摘    要:目的:研究白木香染色体的核型与C-带带型,为该种鉴定、起源、良种培育等的研究提供细胞学资料。方法:核型分析采用常规压片法,C-带带型用改良BSG法。结果:白木香体细胞染色体数目2n=16,核型公式为K(2n)=2x=16=6m 6sm 4st,染色体相对长度组成2n=16=4L 4M2 6M1 2S,属于"2B"型。白木香8对染色体共有34条C-带带纹,C-带带型公式为2n=2x=16=4C 2I 2T 2CI 2CI T 2CI T 2I T 。结论:白木香染色体的不对称性较高,处于较进化地位,为进一步研究提供参考。

关 键 词:白木香  核型分析  C-带
文章编号:1001-4454(2007)07-0762-04
修稿时间:2006-11-072007-01-08

Study on Karyotype and Giemsa C-Banding of Aquilaria sinensis
SHEN Yan-jing,ZHAO Shu-jing.Study on Karyotype and Giemsa C-Banding of Aquilaria sinensis[J].Jorunal of Chinese Medicinal Materials,2007,30(7):762-765.
Authors:SHEN Yan-jing  ZHAO Shu-jing
Institution:1. College of Bioscience and Biotechnology, South China University of Technology, Guangzhou 510641, China; 2. Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China
Abstract:Objective: To study Aquilaria sinensis(Lour.) Spreng chromosome karyotype and C-banding.Method: Sections for karyotype and BSG method for C-banding.Results: The somatic chromosome number was 2n=16,karyotype formula was K(2n)=2x=16=6m 6sm 4st,based on Levan's publication in 1964.According to the method of Kuo,the chromosome based on!the relative length was 2n=16=4L 4M2 6M1 2S,which belonged to "2B".8 pairs chromosomes had 34 C-bands and the C-banding patter was 2n=2x=16=4C 2I 2T 2CI 2CI T 2CI T 2I T .Conclusion: The data of karyotype and C-banding indicated A.sinensis chromosome had a relatively high asymmetry and was in the advanced stage of evolution,which offered the evidence for further genetic analysis.
Keywords:Aquilaria sinensis(Lour  ) Spreng  Karyotype  C-banding
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