| 正交实验法优选骨碎补提取工艺的研究 |
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| 引用本文： | 韦英杰,陈宁,王静,周学敏,杨正华. 正交实验法优选骨碎补提取工艺的研究[J]. 时珍国医国药, 2004, 15(11): 750-751 |
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| 作者姓名： | 韦英杰 陈宁 王静 周学敏 杨正华 |
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| 作者单位： | 1. 江苏省药物研究所,江苏,南京,210009
2. 南京中医药大学实习生,江苏,南京,210009 |
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| 摘 要： | 目的：研究骨碎补的提取工艺。方法：以骨碎补总黄酮及柚皮苷含量为指标，采用正交实验法进行优选，并用紫外分光光度法测定总黄酮的含量．HPLC法测定柚皮苷含量。结果：提取次数是骨碎补有效成分提取的关键因素，其次是溶媒用量．乙醇浓度和提取时间为最次要因素。结论：最佳提取工艺为骨碎补用10倍量60％乙醇回流提取3次．1h/次。
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| 关 键 词： | 骨碎补 正交设计法 提取工艺 总黄酮 柚皮苷 |
| 文章编号： | 1008-0805(2004)11-0750-02 |
| 修稿时间： | 2004-06-07 |
Optimum Extraction on Drynaria fortunei (Kunze) J.Sm.by Orthogonal Design |
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| WEI Ying-jie,CHEN Ning,WANG Jing,ZHOU Xu-min,YANG Zheng-hua. Optimum Extraction on Drynaria fortunei (Kunze) J.Sm.by Orthogonal Design[J]. Lishizhen Medicine and Materia Medica Research, 2004, 15(11): 750-751 |
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| Authors: | WEI Ying-jie CHEN Ning WANG Jing ZHOU Xu-min YANG Zheng-hua |
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| Affiliation: | WEI Ying-jie~1,CHEN Ning~1,WANG Jing~1,ZHOU Xu-min~1,YANG Zheng-hua~2 |
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| Abstract: | Objective: To study the optimum extraction of Drynaria fortunei (Kunze) J. Sm. Methods: Referring to the content of total flavonoids and naringin, we study the optimum extraction by orthogonal HPLC. Results: The cxtraction times is the key factor, the amount of solvent is the second. Conclusion: The optimum extraction is as follows: adding 10 times of 80% ethanol into Drynaria fortunei (Kunze) J. Sm., and boiling for 1 hour each time, extracting three times in all. |
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| Keywords: | Drynaria fortunei (Kunze) J. Sm. Orthogonal design Extraction process Total flavonoids Naringin |
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