倒千里光碱对肝大部分切除小鼠肝脏损伤后再生修复的影响

背景:目前大多数应用倒千里光碱造成肝损伤模型都是以大鼠为实验对象,有研究报道倒千里光碱并不能抑制小鼠肝细胞增殖,也有报道倒千里光碱对小鼠的肝细胞具有增殖抑制作用.目的:观察单纯肝脏大部分切除及其联合应用倒千里光碱致小白鼠对肝损伤后再生修复的影响.方法:40只C57BL/6J小鼠随机数字表法分为2组,每组20只.倒千里光碱/肝脏部分切除组:腹腔注射倒千里光碱溶液70 mg/kg,注射2次,每次间隔2周,4周后肝脏2/3切除.肝脏部分切除组:腹腔注射生理盐水70 mg/kg,注射2次,每次间隔2周,4周后行肝脏2/3切除.观察术后14 d肝脏大体结构恢复情况;苏木精-伊红染色观察术后第3,7天肝细胞损伤情况;BrdU染色观察术后第3天成熟肝细胞增殖情况;CK19和C-kit免疫组织化学方法观察术后第3,7,14天肝脏卵圆细胞增生情况.结果与结论:肝脏部分切除组14 d肝大体结构基本恢复正常,而倒千里光碱/肝脏部分切除组肝脏大小没有明显的恢复.苏木精-伊红染色可见倒千里光碱/肝脏部分切除组明显的肝细胞变性改变;BrdU染色肝脏部分切除组术后第3天有明显肝细胞增殖,而倒千里光碱/肝脏部分切除组肝细胞增殖很少.CK19和C-kit免疫组织化学结果显示倒千里光碱/肝脏部分切除组术后第3天开始肝脏主要通过肝卵圆细胞增生并逐渐向肝细胞和小胆管分化,从汇管区开始向肝小叶内延伸以修复损伤.提示倒千里光碱可抑制小鼠肝脏损伤后肝细胞增殖再生.建立倒千里光碱/肝脏部分切除小鼠模型可诱导肝脏卵圆干细胞增生.

Retrorsine effects on regeneration and repair of injured liver in mice undergoing partial hepatectomy

BACKGROUND:In many studies,rats were commonly used as models of retrorsine-induced hepatic injury.Some reports have confirmed that retrorsine cannot inhibit proliferation of mouse hepatic cells.Other reports have shown that retrorsine has inhibitory effects on proliferation of mouse hepatic cells.OBJECTIVE:To study the liver regeneration after hepatic injury by creating mouse models treated with partial hepatectomy combination with retrorsine.METHODS:A total of 40 C57BL/6J mice were equally and randomly ass...