柴桂安神胶囊治疗失眠的药效学研究

目的研究柴桂安神胶囊对失眠大鼠睡眠时相变化的影响;观察柴桂安神胶囊对小鼠的镇静催眠作用;观察小鼠对催眠阈下催眠剂量戊巴比妥钠作用的影响及对小鼠自主活动的影响。方法利用电刺激诱导大鼠失眠的方法,用脑电图描记,观察给予柴桂安神胶囊后失眠大鼠睡眠时相的变化情况;将小鼠随机分为柴桂安神胶囊低、中、高剂量组(2,4和8g·kg-1),解郁安神冲剂组和空白组,观察给药后入睡潜伏期和睡眠持续时间,观察记录30min内翻正反射消失达1min以上的小鼠数以及末次给药30min后,2min内小鼠自发活动次数。结果柴桂安神胶囊可显著延长失眠大鼠的总睡眠时间(TST)、中、高剂量可明显缩短戊巴比妥钠致小鼠入睡的潜伏时间、延长睡眠持续时间、增加阈下催眠剂量戊巴比妥钠小鼠入睡率并抑制小白鼠自发活动次数。结论柴桂安神胶囊有镇静催眠作用。

Pharmacodynamic study of Chaigui Anshen Capsules in the treatment of insomnia

Objective To study the effect of Chaigui Anshen Capsules on the sleep phase of insomnia rats ;to observe the sedative and hypnotic effects of Chaigui Anshen Capsules on mice ;to observe the influence of subthreshold hypnotic dose of sodium pento-barbital in mice and spontaneous activity in mice after giving Chaigui Anshen Capsules .Methods The influence of Chaigui Anshen Capsules on the sleep phase of insomnia rats induced by electrical stimulation ,was observed and recorded by electroenceghalo-graph（EEG） .The insomnia rats were divided into groups randomly ：Chaigui Anshen Capsules low ,middle and high dose groups（2 , 4 and 8 g · kg -1 ） ,Jieyu Anshen granules group and blank group .After the administration of Chaigui Anshen Capsules ,the sleep latency and sleep duration were observed ,and the number of rats whose loss of righting reflex was more than one minute in 30 mi-nutes .and spontaneous activity times of rats within 2 minute were recorded 30 minutes after the last administration .Results Chaigui Anshen Capsules can significantly prolong the total sleep time（TST） ,while middle and high dose of Chaigui Anshen Cap-sules can shorten the sleep latency induced by pentobarbital sodium ,extend the sleep duration ,increase the sleep rate which treated with pentobarbital sodium and decrease the spontaneous activity times of rats .Conclusion Chaigui Anshen Capsules have the sed-ative and hypnotic effects .