Genetic variability and integration of Merkel cell polyomavirus in Merkel cell carcinoma |
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Authors: | Martel-Jantin C Filippone C Cassar O Peter M Tomasic G Vielh P Brière J Petrella T Aubriot-Lorton M H Mortier L Jouvion G Sastre-Garau X Robert C Gessain A |
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Institution: | a Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USAb Virology Division, United States Army Medical Research Institute for Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA |
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Abstract: | Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with αvβ3 integrin. To study host cell interactions and rapidly test the ability of antibodies to prevent infection, we produced HTNV and PUUV pseudovirions on a vesicular stomatitis virus (VSV) core. Similar to replication-competent hantaviruses, infection was low-pH-dependent. Despite broad cell tropism, several human T cell lines were poorly permissive to hantavirus pseudovirions, compared to VSV, indicating a relative block to infection at the level of entry. Stable expression of αvβ3 integrin in SupT1 cells did not restore infectivity. Finally, the pseudovirion system provided a rapid, quantitative, and specific method to screen for neutralizing antibodies in immune sera. |
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Keywords: | Virus entry Hantavirus Bunyaviridae Glycoprotein Puumala Hantaan Pseudovirions |
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