Characteristics of chemically aggregated IgM in an immunoglobulin class-specific immune complex assay |
| |
| Authors: | H S Margolis O V Nainan H A Fields D W Bradley |
| |
| Institution: | 1. Pacific Meinoke Biopharmaceutical Co, Changzhou, China;2. National Center for Safety Evaluation of Drugs, Beijing, China;3. AbMax Biotechnology Co., LTD, 99 Kechuang 14th St, BDA, Beijing, China;4. Cell Engineering Research Center and Department of Cell Biology, State Key Laboratory of Cancer, Fourth Military Medical University, Xi''an, China |
| |
| Abstract: | Immune complexes have been implicated in the pathogenesis of a number of infectious diseases. The predominant immunoglobulin class associated with circulating immune complexes is IgG, although immune complexes containing IgM have been described. The role of IgM immune complexes in disease pathogenesis has been difficult to characterize due to the lack of a reliable in vitro model. Immunoglobulins aggregated with bis-diazotized benzidine (BDB) are known to function as model immune complexes. We have developed an IgM immune complex using BDB-aggregated IgM which can be used as a reference in a conglutinin-based immune complex assay. Using this assay system, humans and chimpanzees with acute hepatitis A were found to have circulating immune complexes that contained IgM as the predominant antibody. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
