旋毛虫病鼠毒血症的形成与中枢神经系统氧化损伤关系的探讨

目的　探讨旋毛虫病鼠毒血症的形成以及与中枢神经系统氧化损伤的关系。方法　应用黄嘌呤氧化酶（XOD）、丙二醛（MDA）和还原型谷胱甘肽（GSH）3个检测指标从发病学角度分析氧化酶类在宿主重要组织脏器产生的氧化损伤和修复的变化及作用。结果　小鼠肠、肝、心和脑组织中的XOD、MDA和GSH3个检测指标在感染旋毛虫后的不同时期（3～30d）呈现出明显不同的改变。血液中的MDA在感染过程中一直维持较高的水平，并且脑组织中MDA的含量在感染过程中与血液及肠组织中的MDA呈正相关升高。结论　宿主多个脏器组织的氧化损害、氧化代谢毒性产物在机体内的蓄积以及抗氧化能力的改变等因素可能是造成宿主毒血症的形成以及中枢神经系统损害的主要原因。

STUDIES ON RELATIONSHIPBETWEEN FORMATION OF TOXEMIA AND OXIDATIVE DAMAGE OF CENTRAL NERVOUS SYSTEM OF MICE WITHTRICHINOSIS

Objective To study the relationship between formation of toxemia and oxidative damage of central nervous system of mice with trichinosis. Methods The levels and content of Xanthine oxidase(XOD), Malondialdehyde(MDA) and Glutathione(GSH) in the different tissues and sera from mice infected by Trichinella spiralis were determined by those methods introduced in references in the tissues and sera on 3, 7, 14, 30 days post infection. Results In different stages of infection, the levels and content of MDA , XOD and GSH in intestinal, hepatic and cardiac tissue changed significantly than that in control groups. The levels of GSH and MDA in brain tissue showed reverse change from 14 to 30 days, the levels of MDA in the brain rose following MDA in sera and intestine. The level of MDA in sera was steadily maintained at a higher level. Conclusion Oxidative damage of multi organ tissues, accumulation of metabolites and change of anti oxidation in host may be main cause that induce formation of toxemia and oxidative damage of central nervous system of mice with trichinosis.