慢性病毒性肝炎肝细胞凋亡与Fas抗原和病毒表达的相互关系

为探讨病毒性肝炎肝细胞凋亡及其与病毒和Ｆａｓ抗原表达的关系，应用原位末端标记技术检测一组慢性乙、丙型肝炎患者肝组织中细胞凋亡状况，并以免疫组化方法检测病毒和Ｆａｓ抗原表达。结果３１例患者中，２５例肝组织中检出末端标记阳性细胞，散布于肝小叶和肝窦内，部分炎症坏死灶中和胆管上皮细胞也可检出阳性细胞；组织炎症活动度较大者和病毒抗原阳性者凋亡指数较高；凋亡细胞可为病毒抗原阳性和阴性；Ｆａｓ抗原阳性与阴性组间凋亡程度无显著差异。提示病毒性肝炎患者感染和未感染病毒的肝细胞均可发生凋亡，其机制有待进一步研究。

HEPATOCYTE APOPTOSIS IN CHRONIC VIRAL HEPATITIS AND ITS RELATIONSHIP WITH VIRAL AND FAS ANTIGEN EXPRESSION

To study the apoptosis of hepatocytes and its relationship with viral and Fas antigen expression in viral hepatitis, we detected apoptosis of liver cell and the expression of viral and Fas antigens in liver tissues of patients with chronic hepatitis B and/or C virus infection by using in situ end labelling and immunohistochemical methods, respectively. 25 of 31 cases were detected end labelling positive cells in their liver. The stained cells scattered in hepatic lobules, and also located in some necrosis areas and in bile duct epithelium. The apoptosis index was higher in patients with high histological active index (HAI) and in patients with viral antigen(s) positive in their liver than that in patients with low HAI and in patients without viral antigen, respectively. Apoptosis occurred in antigen positive and negative cells. There was no significant difference of apoptosis index between Fas antigen positive and negative group. These results indicated that both the infected and uninfected liver cells could occur apoptosis in patients with viral hepatitis. Further study on the mechanism of liver cell apoptosis is needed.