Castalagin from <Emphasis Type="Italic">Anogeissus leiocarpus</Emphasis> mediates the killing of <Emphasis Type="Italic">Leishmania</Emphasis> in vitro |
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| Authors: | M N Shuaibu K Pandey P A Wuyep T Yanagi K Hirayama A Ichinose T Tanaka I Kouno |
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| Institution: | (1) Natural Product Chemistry, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;(2) Department of Immunogenetics, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan;(3) Animal Research Centre for Tropical Infections, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan;(4) Electron Microscopy Central Laboratory, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan;(5) Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan;(6) Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria |
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| Abstract: | Stem barks of Anogeissus leiocarpus and Terminalia avicennoides widely used in Africa for treatment of some parasitic diseases were collected and made into methanolic extracts. The extracts
were tested on four strains of promastigote forms of Leishmania in vitro. Solvent fractionation in aqueous, butanolic, and ethyl acetate layer indicated butanol and aqueous fractions to
have a superior leishmanicidal activity. Chromatographic separation of the butanolic fraction on Sephadex LH-20 followed by
nuclear magnetic resonance and correlation high-performance liquid chromatography revealed the presence of known hydrolyzable
tannins and some related compounds—with castalagin as the major compound. The observed activity ranged from 62.5 to ≥150,
112.5 to ≥500, and 55 to >150 μg/ml for the crude methanolic extract, different solvent fractions, and the isolated compounds,
respectively, on the four different Leishmania strains. |
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