| 人类细小病毒B19感染致特发性血小板减少性紫癜的发病机制 |
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| 引用本文： | 林雯,金润铭,肖燕,蒙冰,刘朝. 人类细小病毒B19感染致特发性血小板减少性紫癜的发病机制[J]. 实用儿科临床杂志, 2006, 21(15): 983-984 |
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| 作者姓名： | 林雯 金润铭 肖燕 蒙冰 刘朝 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院,儿科,武汉,430022 |
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| 摘 要： | 目的探讨人类细小病毒B19(HPVB19)感染对骨髓巨核祖细胞的影响。方法运用酶联免疫吸附试验检测HPVB19-IgM、PCR检测HPVB19VP独特区DNA。筛选出并HPVB19感染特发性血小板减少性紫癜(ITP)患儿。骨髓行巨核祖细胞体外培养,集落细胞计数。结果外周血HPVB19-DNA阳性及血清HPVB19IgM阳性ITP 31例患儿中,巨核祖细胞集落细胞形成减低,与正常对照组及非HPVB19感染组比较,差异有显著性。结论HPVB19可影响巨核祖细胞集落形成。
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| 关 键 词： | 紫癜 血小板减少性 特发性 类细小病毒B19 人 巨核祖细胞 |
| 文章编号： | 1003-515X(2006)15-0983-02 |
| 收稿时间： | 2006-06-07 |
| 修稿时间： | 2006-06-07 |
Pathogenesis of Idiopathic Thrombocytopenic Purpura Children with Human Parvovirus B19 Infection |
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| LIN Wen,JIN Run-ming,XIAO Yan,MENG Bing,LIU Zhao. Pathogenesis of Idiopathic Thrombocytopenic Purpura Children with Human Parvovirus B19 Infection[J]. Journal of Applied Clinical Pediatrics, 2006, 21(15): 983-984 |
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| Authors: | LIN Wen JIN Run-ming XIAO Yan MENG Bing LIU Zhao |
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| Affiliation: | Department of Pediatrics, Xiehe Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Objective To study the influence of megakaryoblast in bone marrow with the human parvovirus B_(19)(HPVB_(19)) infection in idiopathic thrombocytopenic purpura(ITP).Methods Enzyme linked immunosorbent assay(ELISA) for HPVB_(l9)-IgM and polymerase chain reaction(PCR) for the VP1 of the HPVB_(19) were used.We screened the ITP children with active HPVB_(19) infection,followed by incubation of megakaryoblast in bone marrow in vitro culture and cell counted.Results In 31 of ITP with HPVB_(19)-IgM and HPVB_(19) VP1-DNA,cell colonies were degrade.There were significant difference among normal control group and the group without HPVB_(19) infection.Conclusion HPVB_(19) can influence the forming of megakaryoblast. |
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| Keywords: | purpura thrombocytopenic idiopathic parvovirus B_(19) human megakaryoblast |
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