放射自显影在移植器官低温保存研究中的应用

目的：研究外源笥三磷酸腺苷（adenosine triphosphate,ATP）是否能够进入低温保存大鼠肝脏细胞内。方法：采用大鼠肝脏持续低温机器灌流保存模型，利用放射自显影技术判定α-^32P]ATP是否进入细胞内。结果：灌流液中不加α-^32P]ATP的A组光镜照片显示大鼠肝细胞内外均未见特异性标记银粒显影，而灌流液中含37MBqα-^32P]ATP的B组光镜照片显示大鼠肝细胞内可见多个大小不等的α-^32P]ATP自显影银粒分布，而肝窦及肝小叶中央静脉等血管内则几乎无黑色自显银粒分布。结论：外源性ATP能够进入低温保存大鼠肝细胞内。

Application of Autoradiography in Study of Cold Storaged Transplant Organs

Objective: Our purpose was to study whether exogenous adenosine triphosphate (ATP) could enter liver cell or not. Methods: Using continuous hypothermic machine perfusion model of rat liver, we used autoradiography in this experiment. Results: The light microscope pictures of group A(no ［α-32P］ ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq ［α- 32P］ATP in the perfusate) showed that distribution of numerous ［α-32P］ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel. Conclusion: Exogenous ATP could enter cold storaged rat liver cell.