S型氯代甘油醇对雄性ICR小鼠生育力的影响

目的研究S型氯代甘油醇对雄性ICR小鼠生育能力及睾丸、附睾的组织病理学影响。方法雄性性成熟ICR小鼠64只,随机分为4组,每组16只。实验组分别给予1/40、1/20、1/10LD50(小鼠经口LD50117.54mg/kg)S型氯代甘油醇,对照组给予等体积双蒸水。连续灌胃染毒5天后,每组取4只雄鼠按雌/雄1∶1交配,此后每隔2天交配一次,持续2个月,观察S型氯代甘油醇对雄性小鼠交配指数和雄性生育力指数的影响。实验结束后,每组保留6只动物继续染毒至8个月后进行附睾精子计数和睾丸、附睾的组织病理学检查。结果①与对照组相比,染毒S型氯代甘油醇2个月未引起雄性ICR小鼠交配指数和雄性生育力指数显著降低(P>0.05);②染毒8个月未导致附睾精子数量显著降低(P>0.05),睾丸和附睾的组织病理学检查未见明显异常。结论低剂量长期染毒S型氯代甘油醇对雄性小鼠不具有抗生育作用。

Effects of S-alpha-chlorohydrin on male ICR mice fertility

OBJECTIVE: To study the effects of S-alpha-chlorohydrin on male ICR mice mating index, fertility index and the histopathologic changes of testis and epididymis. METHODS: 64 sexually mature male ICR mice were randomly divided into four groups, each group contained 16 mice. At the doses of 1/40, 1/20 and 1/10LD50 (mice oral, LD50 117.54 mg/kg) S-alpha-chlorohydrin (ACH) were administrated consecutively by oral gavage to male ICR mice, while equal volume redistilled water was given to the controls. At the 5th day mating tests were started, and four male mice from each group were mated with females at a ratio of 1:1. The mating test was carried out every other day until 2 months, and the mating index and the male fertility index induced by S-alpha-chlorohydrin were observed. 6 male mice from each group were exposed with S-alpha-chlorohydrin for another 6 months, and then epididymal cauda sperm counts and histopathological changes in the testes and epididymides were evaluated. RESULTS: (1) In Comparison with control groups, the mating index and male fertility index in male mice exposed to S-alpha-chlorohydrin for two months at any dose level were not changed; (2) There were no significant changes in cauda sperm count between treated and control groups, and no obvious histopathological injuries were observed in testes and epididymides of treated mice. CONCLUSION: Long term exposure to S-alpha-chlorohydrin less than 1/40LD50 did not induce antifertility effects in male ICR mice.