培养细胞的超薄切片技术探讨

目的：探讨培养细胞的电镜良好制样和超薄切片方法。方法：乳腺癌细胞系MB-231培养后分成3组：消化离心组、刮除离心组和原位包埋组，比较培养细胞的超微结构特征。结果：原位包埋方法的超薄切片可以清楚地显现细胞为多角形梭形，细胞连接保持良好。其余两组细胞呈圆形、椭圆形，大部分细胞细胞间连接被破坏，分散分布。结论：原位包埋和单层细胞超薄切片方法是电镜下观察培养细胞的最值得推荐的方法。

Study on ultrathin sectioning technique of the culture cell

Objective: To study on the better methods of specimen preparation and ultrathin sectioning of culture cells by electron microscopy.Methods: Cell line MB-231 of mammary carcinoma were divided randomly into three groups: group of digestive centrifugation, group of cureting centrifugation and group of embedding in situ. The ultrastructural characteristic of the culture cells were compared separately among the three groups.Results: The culture cells of embedding in situ revealed distinctly polygonal cells, spindle cells that cell junction were well retained on ultrathin sectioning. The some cells of other tow groups appeared as round and oval cells. The great majority of cells were scattered, which the cell junctions were destroyed and disappeared.Conclusion: The embedding in situ for culture cell and ultrathin sectioning of the monolayer cell are worth while recommending as a better method for observation by electron microscope.