| Evi1基因特异性siRNA对HEL细胞生长周期的影响 |
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| 引用本文： | 张璞,徐开林,潘秀英,杜冰.Evi1基因特异性siRNA对HEL细胞生长周期的影响[J].江苏医药,2011,37(14). |
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| 作者姓名： | 张璞 徐开林 潘秀英 杜冰 |
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| 作者单位： | 徐州医学院附属医院血液病科,221002 |
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| 基金项目： | 国家自然科学基金项目(30370606) |
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| 摘 要： | 目的研究Evi1基因特异性小干扰RNA(siRNA)对人红白细胞白血病(HEL)细胞增殖及生长周期的影响。方法将HEL细胞分为三组:Evi1基因siRNA组(A组)、siRNA-co对照组(B组)、细胞空白对照组(C组)。转染48 h后,台盼蓝染色实验检测细胞活力,流式细胞术检测细胞周期和凋亡率。结果与B、C组相比,A组HEL细胞活性下降(97.13±1.58)%、(99.20±2.27)%vs.(26.05±2.49)%],细胞周期阻滞在G0/G1期(39.61±1.32)%、(35.77±1.31)%vs.(79.07±1.43)%],S期细胞减少(57.74±1.08)%(、57.36±1.38)%vs.(9.51±0.75)%],凋亡率增高(9.78±1.14)%(、8.67±1.89)%vs.(49.94±1.75)%](P均<0.05);而B组和C组间各观察指标比较差异无统计学意义(P>0.05)。结论 Evi1基因特异性siRNA可抑制HEL细胞增殖,促进细胞凋亡。
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| 关 键 词： | 小干扰RNA Evi1基因 人红白细胞白血病细胞 |
Effect of specific siRNA of Evi1 gene on the growth cycle of HEL cells |
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| ZHANG Pu,XU Kaili,PANG Xiuying,et al..Effect of specific siRNA of Evi1 gene on the growth cycle of HEL cells[J].Jiangsu Medical Journal,2011,37(14). |
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| Authors: | ZHANG Pu XU Kaili PANG Xiuying |
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| Institution: | ZHANG Pu,XU Kaili,PANG Xiuying,et al.Department of Hematology,Affiliated Hospital,Xuzhou Medical College,Xuzhou 221002,CHINA |
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| Abstract: | Objective To investigate the effect of specific small interfering RNA(siRNA) of Evi1 gene on the proliferation and growth cycle of human erythroleukemia(HEL) cells.Methods The HEL cells were devided into three groups of A(transfected with Evi1 siRNA),B(transfected with siRNA-co) and C(blank controls).At 48 h after the transfection,cell activity was detected by trypan blue dye test,and cell cycle and rate of cell apoptosis were analyzed by flow cytometry.ResultsCompared with groups of B and C,cell activity w... |
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| Keywords: | Small interfering RNA Evi1 gene Human erythroleukemia cells |
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