Inhibition of KL-6/MUC1 glycosylation limits aggressive progression of pancreatic cancer |
| |
Authors: | Huan-Li Xu;Xin Zhao;Ke-Ming Zhang;Wei Tang;Norihiro Kokudo; |
| |
Institution: | Huan-Li Xu, Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, ChinaXin Zhao, Ke-Ming Zhang, Department of Hepatobiliary Surgery, 302 Hospital of Chinese People’s Liberation Army, Beijing 100039, ChinaWei Tang, Norihiro Kokudo, Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, University of Tokyo, Tokyo 113-8654, Japan |
| |
Abstract: | AIM: To evaluate the significance of KL-6/MUC1 (a type of MUC1) glycosylation in pancreatic cancer progression.METHODS: KL-6/MUC1 expression was detected by immunohistochemistry in 48 patients with pancreatic duct cell carcinoma. The N-/O-glycosylation inhibitors (tunicamycin and benzyl-N-acetyl-α-galactosaminide) were then used to interfere with KL-6/MUC1 glycosylation in two pancreatic carcinoma cell lines, and the effects on KL-6/MUC1 expression, and cell adhesion and invasion were determined. In addition, protein expression of epithelial-mesenchymal transition markers, E-cadherin and vimentin, were evaluated in cells after treatment with glycosylation inhibitors.RESULTS: Overexpression of KL-6/MUC1 was found in all pancreatic cancer tissues, but not in the surrounding normal pancreatic tissues. The expression profile of KL-6/MUC1 was significantly decreased after treatment with the inhibitors. The adhesion and invasive ability of cancer cells were significantly decreased after drug treatment, and increased E-cadherin and decreased vimentin expression were found.CONCLUSION: KL-6/MUC1 glycosylation is involved in pancreatic cancer metastasis and invasion. Therapeutic strategies which target this may help control the aggressive behavior of pancreatic cancer cells. |
| |
Keywords: | Benzyl-N-acetyl-α-galactosaminide Glycosylation KL-6/MUC1 Pancreatic carcinoma Tunicamycin |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《世界胃肠病学杂志(英文版)》浏览原始摘要信息 |
|