Fucoidan, a sulfated polysaccharide from brown algae, against myocardial ischemia-reperfusion injury in rats via regulating the inflammation response |
| |
Authors: | Chunmei LiYonglin Gao Yanli XingHaibo Zhu Jingyu ShenJingwei Tian |
| |
Institution: | a School of Pharmacy, Yantai University, Yantai 264005, PR China b Science and Engineering College of Chemistry and Biology, Yantai University, Yantai 264005, PR China c Shandong Luye Research and Development for Natural Drugs Co. Ltd., Yantai 264003, PR China |
| |
Abstract: | The aim of the study was to determine the effects of fucoidan on rat myocardial ischemia-reperfusion (I/R) model and elucidate the potential mechanisms. Myocardial I/R injury was induced by the occlusion of left anterior descending coronary artery for 30 min followed by reperfusion for 2 h. After 2 h reperfusion, hemodynamics parameters were detected. Blood samples were collected to determine serum levels of tumor necrosis factor-α (TNF-α) and interleukin 6, 10 (IL-6, 10). Hearts were harvested to assess histopathological changes, infarct size (IS), and the content of myeloperoxidase (MPO). The expression of high-mobility group box 1 (HMGB1), phosphor-IκB-α and phosphor-nuclear factor kappa B (NF-κB) were assayed by western blot. Compared with control group, treatment with fucoidan improved left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and the contractility index (P < 0.05, P < 0.01). Fucoidan reduced the myocardial IS, the levels of TNF-α and IL-6, and the activity of MPO (P < 0.05, P < 0.01). Fucoidan down-regulated the expression of HMGB1, phosphor-IκB-α and NF-κB, but increased the content of IL-10 when compared with control (P < 0.05, P < 0.01). Besides, the infiltration of polymorph nuclear leukocytes (PMNs) and histopathological damages in myocardium were decreased in fucoidan treated groups (PMNs, P < 0.05, P < 0.01). These findings revealed that the administration of fucoidan could regulate the inflammation response via HMGB1 and NF-κB inactivation in I/R-induced myocardial damage. |
| |
Keywords: | BCA bicinchoninic acid HR heart rate H& E hematoxylin-eosin HMGB1 high-mobility group box 1 IL-6 interleukin 6 IS infarct size LAD left anterior descending LVEDP left ventricular end-diastolic pressure LVSP left ventricular systolic pressure MAP mean artery pressure MPO myeloperoxidase NF-κB nuclear factor kappa B PMNs polymorph nuclear leukocytes TTC triphenyltetrazolium chloride ROS reactive oxygen species TNF-α tumor necrosis factor-α |
本文献已被 ScienceDirect 等数据库收录! |
|