| KLF6在肝细胞癌中的表达及在肝癌细胞系中对增殖和凋亡的影响 |
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| 引用本文： | 梁明,吕淑兰,王晓燕,李静媛,赵勇华,康鹏,高杰.KLF6在肝细胞癌中的表达及在肝癌细胞系中对增殖和凋亡的影响[J].肝脏,2011,16(1):31-34. |
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| 作者姓名： | 梁明 吕淑兰 王晓燕 李静媛 赵勇华 康鹏 高杰 |
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| 作者单位： | 哈尔滨医科大学附属第二医院感染科,150086 |
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| 基金项目： | 黑龙江省卫生厅科研课题(2007-367) |
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| 摘 要： | 目的 检测抑癌基因KLF6在人肝细胞癌中的表达情况,探讨KLF6在肝癌细胞系中对细胞增殖及凋亡的影响.方法 分别用荧光定量PCR、RT-PCR 和Western blot 、免疫组织化学方法检测肝细胞癌、癌旁组织及正常肝组织中KL F6 基因mRNA 及蛋白水平的表达情况.构建KLF-6基因的真核表达质粒,用MTT法检...
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| 关 键 词： | 肝细胞癌 Kruppel样因子6 凋亡 |
The expression of KLF-6 in HCC and its effect on proliferation and apoptosis in HCC cell lines |
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| LIANG Ming,LV Shu-lan,WANG Xiao-yan,LI Jing-yuan,ZHAO Yong-hua,KANG Peng,GAO Jie.The expression of KLF-6 in HCC and its effect on proliferation and apoptosis in HCC cell lines[J].Chinese Hepatology,2011,16(1):31-34. |
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| Authors: | LIANG Ming LV Shu-lan WANG Xiao-yan LI Jing-yuan ZHAO Yong-hua KANG Peng GAO Jie |
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| Institution: | LIANG Ming,LV Shu-lan,WANG Xiao-yan,LI Jing-yuan,ZHAO Yong-hua,KANG Peng,GAO Jie.Department of Infectious Diseases,Second Hospital of Harbin Medical University,Harbin 150086,China |
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| Abstract: | Objective To detect the expression of KLF-6,a tumor suppressor gene,in human hepatocellular carcinoma and to explore its role in proliferation and apoptosis in HCC cell lines.Methods The expression of KFL6 mRNA and protein were tested by Real Time PCR,immunohistochemistry and Western blot respectively in hepatocellular carcirnoma tissues,and paracancerous tissues and normal liver tissues.Corresponding plasmid of KLF6 was conducted,and proliferation of target cells was measured with MTT.Results Compared with... |
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| Keywords: | Hepatocellular carcinoma KLF6 Apoptosis |
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