| 肝纤维化小鼠肝组织血管新生特点及其形成机制 |
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| 引用本文： | 吕靖,陆雄,陶艳艳,赵志敏,刘成海.肝纤维化小鼠肝组织血管新生特点及其形成机制[J].肝脏,2011,16(1):35-40. |
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| 作者姓名： | 吕靖 陆雄 陶艳艳 赵志敏 刘成海 |
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| 作者单位： | 1. 上海市中医临床重点实验室,上海中医药大学附属曙光医院肝病研究所,201203
2. 上海中医药大学科技实验中心 |
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| 基金项目： | 国家自然科学基金(30901943); 上海市优秀学科带头人计划项目(08XD14041); 国家重点基础研究发展计划(973)项目(2006CB504801); 上海市教育委员会E-研究院建设计划项目(E0308); 上海市教委创新团队建设项目 |
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| 摘 要： | 目的 探讨肝纤维化小鼠肝组织血管新生特点及其形成机制.方法 C57BL/6小鼠随机分为正常对照组与模型18 h、4周、8周组,共4组.采用CCl4诱导小鼠肝纤维化模型.天狼猩红染色观察肝组织胶原沉积;免疫荧光检测肝组织vWF蛋白表达,分析微血管密度;扫描电镜及透射电镜观察肝窦内皮结构;Western blot法检测肝组...
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| 关 键 词： | 肝窦毛细血管化 血管新生 HIF1α |
The characteristic and mechanism of angiogenesis in a mouse model of liver fibrosis |
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| LU Jing,LU Xiong,TAO Yan-yan,ZHAO Zhi-min,LIU Cheng-hai.The characteristic and mechanism of angiogenesis in a mouse model of liver fibrosis[J].Chinese Hepatology,2011,16(1):35-40. |
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| Authors: | LU Jing LU Xiong TAO Yan-yan ZHAO Zhi-min LIU Cheng-hai |
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| Institution: | LU Jing,LU Xiong,TAO Yan-yan,ZHAO Zhi-min,LIU Cheng-hai Institute of Liver Diseases,ShuGuang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China |
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| Abstract: | Objective To investigate the characteristic and mechanism of liver angiogenesis in a mouse model of liver fibrosis.Methods Forty-nine male C57BL/6 mice,were randomly divided into four groups: normal(n=10),model 18 h(n=13),model 4 wk(n=13),model 8 wk(n=13).The model group was given 50% CCl4-olive oil subcutaneously injection.Sirius red staining was used to detection of hepatic collagen deposition;immunofluorescence assay for vWF was performed to analyze hepatic microvessel density;sinusoidal endothelial stru... |
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| Keywords: | Hepatic sinusoidal capillarization Angiogenesis HIF1α |
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