Zinc-dependent cytoadherence of Legionella pneumophila to human alveolar epithelial cells in vitro |
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| Affiliation: | 1. Université de Lyon, Lyon F-69008, France;2. Université Lyon 1, Faculté Laennec, Lyon F-69008, France;3. Centre National de Référence des Légionelles, INSERM U851, Faculté de Médecine Laennec, Laboratoire de Bactériologie, 7 rue Guillaume-Paradin, 69372 Lyon cedex 08, France;4. Hospices Civils de Lyon, Centre de Biologie et Est, Bron F-69677, France;5. Unité INSERM U629, Mécanismes Moléculaires de la Pathogenèse Bactérienne, Institut Pasteur de Lille, 1 rue Pr. Calmette, 59019 Lille, France;1. Laborelec, Rodestraat 125, 1630 Linkebeek, Belgium;2. E.ON Technologies GmbH, Alexander-von-Humboldt-Str. 1, D 45896 Gelsenkirchen, Germany;3. TNO, Leeghwaterstraat 46, Delft 2628CA, The Netherlands;4. Karlsruhe Institute of Technology (KIT), Institut für Technische Thermodynamik und Kältetechnik, Engler-Bunte-Ring 21, 76131 Karlsruhe, Germany;1. Soil and Water Laboratory, Department of Civil and Environmental Engineering, Rensselaer Polytechnic Institute, 110 8th street, Troy, NY 12180, USA;2. Department of Natural Resources, Cornell University, Ithaca, NY 14850, USA;1. Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China;2. Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China;3. Wenzhou Environmental Protection Design Scientific Institute, Wenzhou 325000, China |
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| Abstract: | Microbial adherence to host cells is an early key step in the establishment of infection. During the course of Legionnaire's disease, Legionella interactions with host cells are best documented for resident macrophages. However, L. pneumophila can also replicate within type I and type II pneumocytes, which cover almost the entire alveolar surface. In the presence of zinc, we observed a significant and concentration-dependent increase in L. pneumophila adherence to and invasion of type II pneumocytes. The zinc-dependent adherence mechanism seemed to be host-cell-independent, as a similar increase in cytoadherence was observed with macrophages. We also found that zinc-dependent adherence of L. pneumophila appears to involve recognition of zinc-binding pneumocyte receptors by a bacterial adhesin, and heparan-sulfated host cell receptors, but not type IV pili. |
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