| HPLC法同时测定丹参中原儿茶醛、迷迭香酸、丹酚酸A的含量 |
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| 引用本文： | 王招玉,徐依萍,焦蓉,赵娜,李将,李鹏,陈琴华. HPLC法同时测定丹参中原儿茶醛、迷迭香酸、丹酚酸A的含量[J]. 中国药师, 2014, 0(9): 1473-1475 |
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| 作者姓名： | 王招玉 徐依萍 焦蓉 赵娜 李将 李鹏 陈琴华 |
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| 作者单位： | 湖北医药学院附属东风医院实验中心 湖北十堰 442008；湖北医药学院药学院;湖北医药学院药学院;湖北医药学院药学院;湖北医药学院药学院;湖北医药学院药学院;湖北医药学院附属东风医院实验中心 湖北十堰 442008；湖北医药学院药学院;湖北医药学院附属东风医院实验中心 湖北十堰 442008；湖北医药学院药学院 |
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| 基金项目： | 十堰市科技局项目(编号：2013st033)；湖北省大学生创新训练项目（编号：201310929025）;湖北省2011协同创新中心基金（项目编号：4） |
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| 摘 要： | 目的：建立用HPLC同时测定丹参药材中丹酚酸A,原儿茶醛,迷迭香酸含量的方法。方法：采用色谱柱为LinksilODS色谱柱（250 mm×4.6 mm,5μm）;流动相为1%的冰醋酸水溶液∶乙腈=7∶3;流速为0.8 ml·min-1;检测波长为280 nm;柱温为30℃,进样量20μl。结果：丹酚酸A、原儿茶醛、迷迭香酸的线性范围均为3~300μg·ml-1（r〉0.999 0）,加样回收率在95.22%~99.68%之间,RSD均小于2%（n=5）。结论：该方法快速准确,重复性好,可用于丹参药材的质量控制。
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| 关 键 词： | 丹参 原儿茶醛 迷迭香酸 丹酚酸A 含量测定 高效液相色谱法 |
| 收稿时间： | 2014-01-30 |
| 修稿时间： | 2014-04-08 |
Simultaneous Determination of Protocatechualdehyde,Rosmarinic Acid and Salvianolic Acid A in Salvia Miltiorrhiza Bunge by HPLC |
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| Wang Zhaoyu,Xu Yiping,Jiao Rong,Zhao N,Li Jiang,Li Peng and Chen Qinhua. Simultaneous Determination of Protocatechualdehyde,Rosmarinic Acid and Salvianolic Acid A in Salvia Miltiorrhiza Bunge by HPLC[J]. China Pharmacist, 2014, 0(9): 1473-1475 |
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| Authors: | Wang Zhaoyu Xu Yiping Jiao Rong Zhao N Li Jiang Li Peng Chen Qinhua |
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| Affiliation: | Medical Experimental Center, Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei Shiyan 442008, China; Hubei University of Medicine;Hubei University of Medicine;Hubei University of Medicine;Hubei University of Medicine;Hubei University of Medicine;Medical Experimental Center, Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei Shiyan 442008, China; Hubei University of Medicine;Medical Experimental Center, Affiliated Dongfeng Hospital, Hubei University of Medicine, Hubei Shiyan 442008, China; Hubei University of Medicine |
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| Abstract: | Objective： To establish an HPLC method to simultaneously determine the content of protocatechualdehyde,rosmarinic acid and salvianolic acid A in Salvia miltiorrhiza Bunge. Methods： The chromatographic column was a Linksil-ODS（ 250 mm ×4. 6 mm,5 μm） column,the mobile phase was 1% acetic acid water solution-acetonitrile（ 7∶ 3） with the flow rate of 0. 8 ml·min- 1.The detection wavelength was 280 nm,the column temperature was 30℃ and the injection volume was 20 μl. Results： The linear range of protocatechualdehyde,rosmarinicacid and salvianolic acid A was 3-300 μg·ml- 1（ r 0.999 0）,and the recovery was within the range of 95. 22%-99. 68% with RSD below 2%（ n = 5）. Conclusion： The method is accurate,rapid and reproducible,and can be used to control the quality of Salvia miltiorrhiza Bunge. |
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| Keywords: | Salvia miltiorrhiza Bunge Protocatechualdehyde Rosmarinic acid Salvianolic acid A Determination HPLC |
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