人参总皂苷联合淫羊藿苷对血管性痴呆大鼠学习记忆及海马神经细胞凋亡的影响研究

目的：研究人参总皂苷联合淫羊藿苷对血管性痴呆大鼠学习记忆、氧自由基及海马CA1区神经细胞凋亡的影响。方法：采用反复夹闭双侧颈总动脉再灌注同时腹腔注射硝普钠方法建立血管性痴呆大鼠模型,连续给药21d后,用八臂电迷宫法判断给药前后大鼠的学习和记忆能力,再进行脑组织SOD、MDA的测定和海马CA1区神经细胞凋亡检测。结果：与假手术组大鼠相比,模型大鼠电迷宫学习和记忆错误次数明显增加（P〈0.05）,大鼠造模前后连续灌胃给药21 d后,大鼠上述行为学指标得到明显改善（P〈0.05）,脑组织SOD活性提高（P〈0.05）和MDA含量降低（P〈0.01）,海马CA1区神经细胞凋亡数减少（P〈0.05）。结论：人参总皂苷联用淫羊藿苷对血管性痴呆大鼠学习记忆障碍有显著的预防和治疗作用,可能与通过改善血管性痴呆大鼠大脑自由基代谢和海马CA1区神经细胞损伤有关,从而起到保护大脑的作用。

Effects of Total Saponins of Panax Ginseng Combined with Icariin on Learning, Memory and Apoptosis of Hippocampal Neural Cells in Vascular Dementia Rats

ABSTRACT Objective: To explore the effects of total saponins of panax ginseng (TSPG) combined with icariin (ICA) on learning and memory capability, oxygen free radical and apoptosis of hippocampal CA1 pyramidal cells in vascular dementia (VD) rats. Methods:The VD rats were obtained by occlusion of bilateral common carotid artery and reperfusion repeatedly combined with intraperitoneal injection of sodium nitroprusside. Spacial learning and memory ability was evaluated by 8-arm radial electric maze test on the 21st day after the drug treatment, the activity of SOD and the level of MDA in the brain were determined, and the adjacent sections were used to detect fragmented DNA in apoptotic cells(TUNEL). Results:The learning and memory ability of the model rats judged by the 8-arm radial electric maze test was significantly decreased compared with that in the sham operated group (P<0.05). The combination treatment could significantly protect and improve all the evaluated indices of the model rats after the 3-week treatment (P<0.05) with increased SOD (P<0.05), and decreased MDA (P<0.01) and TUNEL positive cells (P<0.05). Conclusion:The combination drug treatment can significantly protect and improve the spacial learning and memory ability of VD rats, which is related with such effects as improving oxygen free radical metabolism and nerve cell injure in hippocampal CA1 region.