HPLC法测定参蛇软膏中盐酸小檗碱的含量

目的：建立参蛇软膏中盐酸小糪碱的含量测定方法。方法：采用高效液相色谱法测定参蛇软膏中盐酸小糪碱的含量,Kromasil C18色谱柱（250 mm×4.6 mm,5μm）;乙腈-0.1%磷酸（41∶59）（每100 ml加入十二烷基磺酸钠0.02 g）为流动相;流速：1.0 ml·min-1;检测波长：320 nm;柱温：室温;进样量：20μl。结果：盐酸小檗碱与其他组分分离良好,在0.059 2~0.296 0 g·L-1范围内线性关系良好（r=0.999 4）;平均加样回收率为99.80%,RSD=0.24%（n=6）。结论：该方法简单、专属性强、重复性好,可用于参蛇软膏的含量测定。

Determination of Berberine Hydrochloride in Shenshe Ointments by HPLC

Objective： To establish the determination method for berbefine hydrochlofide in Shenshe ointments. Methods： The quantitative analysis of berberine hydrochloride in Shenshe osintments was carried out by HPLC with a Kromsal C18 chromatographic column （250 mm ×4.6 mm, 5 μm）, the mobile phase was acetonitrile-0.1% phosphoric acid （49：51 ） （adding 0.2g sodium dodecylsulphate into 100 ml solution） with the flow rate of 1.0 ml · min^- 1 and the detection wavelength of 320nm, the temperature of column was room temperature, and the injection volume was 20 I＆l. Results： The linear range was 0. 059 2-0. 296 0 g · L^- 1 with good correlation（r =0.999 4）. The average recovery was 99.80% and RSD was 0.24% （n =6）. Conclusion： The established method is accurate, specific and reproducible, and suitable for the determination of berberine hydrochloride in Shenshe ointments.