灵芪蠲肝液对大鼠TLR4信号途径的影响

目的：探讨四氯化碳（CCl4）诱导大鼠肝损伤的作用机制,并观察灵芪蠲肝液对Toll样受体4（TLR4）信号传导途径的影响。方法：雄性健康Wistar大鼠,设置模型组、灵芪蠲肝组（治疗组）和空白组。用40%CCl4花生油溶液皮下注射建立慢性肝损伤模型,以灵芪蠲肝液灌胃作治疗性研究,于第8周收集血液和肝脏组织。检测肝功能ALT、AST、白蛋白（ALB）水平;检测血清内毒素、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）水平;分离枯否细胞（KCs）,采用逆转录-聚合酶链式反应（RT-PCR）检测KCs TLR4 mRNA表达;肝组织HE染色,按Knodell HAI评分标准对肝脏炎症评分,免疫组化SP法检测肝组织TLR4和核因子κB（NF-κB）蛋白表达。结果：空白组除ALB高于模型组和治疗组外,其余指标均最低。模型组血清ALT、AST、内毒素、IL-1β、TNF-α水平明显高于治疗组（P〈0.001）,ALB水平明显低于治疗组（P〈0.05）,KCs TLR4 mRNA表达明显高于治疗组（P〈0.001）,肝组织炎症Knodell HAI评分、TLR4和NF-κB蛋白均高于治疗组（P〈0.001）。结论：①通过TLR4信号传导途径释放炎症介质致肝组织损伤可能为CCl4诱导慢性肝损伤的机制之一。②灵芪蠲肝液治疗慢性肝损伤的机制可能与抑制TLR4信号途径有关。

THE EFFECT OF LINGQIJUANGAN LIQUOR ON TOLLLIKE RECEPTOR 4 SIGNALING IN RATS WITH CHRONIC LIVER INJURY

Objective: To investigate the mechanism of chronic liver injury induced by CCl4,and to observe the role of TLR4 signaling in mechanism of chronic liver injury.Methods:Male wistar rats were randomly divided into normal control group,model group and Lingqijuangan group(therapeutic group).The chronic liver injury was induced by hypodermic injection of 40% CCl4,and the therapeutic group was given Lingqijuangan Liquor by intragastric administration.Blood and liver were collected at the same time at the end of th...