布地奈德对肺纤维化大鼠的影响

THE EFFECT OF BUDESONIDE ON RATS WITH PULMONARY FIBROSIS

Objective： To investigate the effect of Budesonide（BUD） on type Ⅰand Ⅲ collagen mRNA in lung tissue and TNF-α,TGF-β1,PDGF-BB,IFN-γ in BALF in rats with pulmonary fibrosis.Methods： 45 adult female Wistar rats were randomly divided into normal saline（NS） group,BUD group and bleomycin（BLM） group.Both BUD group and BLM group were instilled into the tracheas with BLM,and NS group with 0.9 % NaCl solution.Aerosol inhalation was performed in 3 groups once everyday at day 0～6（NS group and BLM group inhaled aerosolized NS;BUD group inhaled aerosolized BUD）.5 rats in every group were sacriced at 7th,14th,28th day.The extent of alveolitis and fibrosis was observed in HE and Masson staining.The levels of type Ⅰ/ Ⅲcollagen mRNA in lung tissue were measured by RT-PCR.The concentration of TNF-α,TGF-β1,PDGF-BB,IFN-γ in BALF was measured by ELISA.Results： The extent of alveolitis in BUD group at 7th,14th day was significantly lower than those of BLM group（P〈0.05）.The extent of fibrosis in lung tissue and the concentration of TNF-α,TGF-β1 and PDGF-BB in BALF in BUD group at 7th,14th,28th day was significantly lower than those in BLM group（P〈0.05）;The levels of type Ⅰ/ Ⅲcollagen mRNA at 7th,14th,28th day in BUD group was significantly lower than those in BLM group（P〈0.05）.However,the concentration of IFN-γ in BUD group at 7th,14th and 28th day was significantly higher than those of BLM group（P〈0.05）.Conclusion： After inhaled BUD,the levels of type Ⅰ/Ⅲ collagen mRNA in lung tissue and the concentration of TNF-α、TGF-β1、PDGF-BB in BALF could be decreased,the concentration of IFN-γ in BALF could be increased,and the formation of pulmonary fibrosis could be suppressed in bleomycin-induced pulmonary fibrosis rats.