| 蜕皮甾酮对高糖培养大鼠肾小球系膜细胞增殖及其细胞外基质含量的影响 |
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| 引用本文： | 高岑,马跃荣,史书龙,陈秋.蜕皮甾酮对高糖培养大鼠肾小球系膜细胞增殖及其细胞外基质含量的影响[J].泸州医学院学报,2011,34(4):330-333. |
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| 作者姓名： | 高岑 马跃荣 史书龙 陈秋 |
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| 作者单位： | 1. 泸州医学院病理教研室,四川 泸州,646000
2. 成都中医药大学附属医院内分泌科 |
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| 基金项目： | 四川省重大攻关及应用基础项目(2006J13-033) |
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| 摘 要： | 目的：观察蜕皮甾酮对高糖环境下大鼠肾小球系膜细胞（MCs）增殖、细胞外基质的影响。方法：体外培养大鼠MCs细胞株,分低糖组、高糖组、高糖＋蜕皮甾酮组（EDS组）,分别作用12、24、48h,MTT法检测细胞增殖活力,ELISA法检测细胞上清中FN的含量。结果：与低糖组比较,高糖组12h、24h、48h MCs增殖显著增快（P〈0.01）,FN含量明显增加（P〈0.05）;与低糖组比较,EDS在浓度〉1×10-5mol/L时MCs增殖显著减慢（P〈0.01）;与高糖组比较,EDS 1×10-6mol/L组各时间点及1×10-7mol/L组24h、48h MCs增殖显著减慢（P〈0.05或P〈0.01）,EDS 1×10-6mol/L组各时间点FN含量均有降低（P〈0.05或P〈0.01）;EDS 1×10-10、1×10-8mol/L组各时间点FN含量差异无统计学意义（P〉0.05）。结论：①蜕皮甾酮在浓度〉1×10-5mol/L时对MCs有明显的细胞毒性作用。②蜕皮甾酮可抑制高糖诱导的MCs增殖。③蜕皮甾酮能减少高糖培养大鼠MCs细胞外基质的增加。
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| 关 键 词： | 蜕皮甾酮 糖尿病肾病 肾小球系膜细胞 细胞外基质 |
EFFECTS OF ECDYSTERONE ON PROLIFERATION OF MESANGIAL CELLS AND CONCENTRATION OF EXTRACELLULAR MATRIX IN HIGH GLUCOSE CULTURED RAT MESANGIAL CELLS |
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| Gao Cen,MA Yuerong,SHI Shulong,CHEN Qiu.EFFECTS OF ECDYSTERONE ON PROLIFERATION OF MESANGIAL CELLS AND CONCENTRATION OF EXTRACELLULAR MATRIX IN HIGH GLUCOSE CULTURED RAT MESANGIAL CELLS[J].Journal of Luzhou Medical College,2011,34(4):330-333. |
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| Authors: | Gao Cen MA Yuerong SHI Shulong CHEN Qiu |
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| Institution: | Gao Cen,et al Department of Pathology,Luzhou Medical College |
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| Abstract: | Objective: To investigate the effects of ecdysterone on proliferation of mesangial cells(MCs) and concentration of extracellular matrix in high glucose cultured rat MCs.Methods: Rat MCs were divided into low glucose group,high glucose group and high glucose+ecdysterone group(EDS group which was subdivided according to different final concentration of ecdysterone),and were cultured for 12h,24h and 48h.At indicated time points,cellular proliferation was assessed by MTT,the levels of fibronectin in supernatant... |
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| Keywords: | Ecdysterone Diabetic nephropathy Mesangial cells Extracellular matrix |
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