氧化低密度脂蛋白循环免疫复合物诱导巨噬细胞胆固醇酯蓄积

目的:探讨氧化、天然低密度脂蛋白循环免疫复合物(Ox -LDL- IC)致动脉粥样硬化作用。　方法:采用人源的氧化、天然LDL与异源的抗载脂蛋白B(apoB)结合制备IC,观察不同浓度的Ox- LDL -IC对小鼠腹腔巨噬细胞胆固醇酯蓄积和泡沫细胞形成的影响。　结果:氧化、天然LDL- IC均可引起巨噬细胞内胆固醇酯大量堆积,其效果强于Ox -LDL(P<0. 001),而且随剂量的增加而升高,并转化为泡沫细胞;而天然LDL、抗apoB处理的巨噬细胞内未见胆固醇酯的堆积。　结论:LDL- IC可导致巨噬细胞转化为泡沫细胞,参与动脉粥样硬化的形成。

Oxidized low-density lipoprotein immune complexes induce cholesteryl ester accumulation inhuman macrophages

Objective:To investigate the atherogenic role of oxidized and native LDL immune complexes (IC). Methods:LDL-IC were prepared by using human oxidized and native LDL interaction with sheep antibodies against human apolipoproteinB, respectively. And the effect of oxidzed LDL-IC inducing cholesteryl ester(CE) accumulation in human macrophages was studied. Results:Oxidized and native LDL-ICboth induced cholesteryl ester accumulation in human macrophages, the level of cellular CE induced by IC were significantly higher than the corresponding level induced by oxidized LDL (P< 0.001).Furthermore, the macrophages were found translated into foams. The degree of cholesteryl ester accumulation in macrophages improved, along with oxidized LDL-IC level increasing. Whereas native LDL and apoB-IgG did not show any effect on the level of cellular CE. Conclusion:LDL-IC translate macrophages into foams and partcipate in atherosclerotic formation.