| 氧浓度对关节软骨细胞体外培养的影响 |
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| 引用本文： | 吴华,吴纪饶,王曼莹. 氧浓度对关节软骨细胞体外培养的影响[J]. 医学综述, 2006, 12(8): 454-456 |
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| 作者姓名： | 吴华 吴纪饶 王曼莹 |
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| 作者单位： | 1. 江西师范大学体育学院,江西,南昌,330027
2. 江西师范大学生命科学学院,江西,南昌,330027 |
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| 摘 要： | 关节软骨在体内属缺氧组织,且组织内氧浓度水平呈现梯度,表现为软骨组织表层为10%,而至深层则下降到1%,因此采用空气中20%氧浓度对软骨细胞进行体外培养,显然不适宜。通过对关节软骨组织的生物学特性、生存环境以及不同氧浓度下软骨细胞体外培养的实验研究分析,认为5%氧浓度是体外培养关节软骨细胞的适宜浓度,同时在软骨细胞的不同生长时期对培养基氧浓度进行控制,可将软骨细胞处于较好的生长增殖状态。
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| 关 键 词： | 软骨细胞 体外培养 氧浓度 |
| 文章编号： | 1006-2084（2006）08-0454-03 |
| 收稿时间： | 2005-05-29 |
| 修稿时间： | 2006-01-30 |
The Influence of Oxygen Concentration on Articular Chondrocytes Cultured in Vitro |
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| WU Hua,WU Ji-rao,WANG Man-ying. The Influence of Oxygen Concentration on Articular Chondrocytes Cultured in Vitro[J]. Medical Recapitulate, 2006, 12(8): 454-456 |
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| Authors: | WU Hua WU Ji-rao WANG Man-ying |
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| Affiliation: | 1. Jiangxi Normal University Physical Education Institute, Nanchang 330027, China ; 2. Jiangxi Normal University Life Science Institute, Nanchang 330027, China |
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| Abstract: | Articular cartilage is a physiologically hypoxie tissue with a proposed gradient of oxygen tension ranging from about 10% oxygen at the cartilage surfane to less than 1% in the deepest layers.Therefora 20% 02 ,being unphysiological, may not be optimal for the culture of chondtocytes in vitro This article analysis the biological character and survivable environment of chondrocytes in vivo and analysis the study results about chondrocyte cultured under different oxygen concentration in vitro,which suggest that oxygen concentration about 5% O2 is optimal for the culture of chondrocytes in vitro and it is better for its prolification by controlling oxygen concentration during its growth periods. |
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| Keywords: | Chondrocyte In vitro Oxygen concentration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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