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| 表阿霉素脂质体的制备与质量评价 | |
| 引用本文： | 吴燕,吴诚,康艳敏,梅兴国.表阿霉素脂质体的制备与质量评价[J].军事医学科学院院刊,2008,32(2):165-167. |
| 作者姓名： | 吴燕 吴诚 康艳敏 梅兴国 |
| 作者单位： | 1. 军事医学科学院毒物药物研究所,北京,100850 2. 第二炮兵总医院药剂科,北京,100088 3. 延边大学药学院,吉林延吉,133000 |
| 摘 要： | 目的:制备盐酸表阿霉素脂质体,建立HPLC含量测定方法并优选包封率测定方法.方法:采用薄膜分散-pH梯度法制备盐酸表阿霉素脂质体;采用HPLC测定药物含量;采用葡聚糖凝胶色谱、超滤及透析3种分离方法测定脂质体的包封率.结果:制备的表阿霉素脂质体粒径较小、分布较窄;3种方法测得盐酸表阿霉素脂质体的包封率分别为96.1%,98.4%,92.9%;结论:薄膜分散-pH梯度法适于制备盐酸表阿霉素脂质体,含量测定方法可靠.超滤法可以准确、快速地测定脂质体包封率. |
| 关 键 词： | pH梯度法 盐酸表阿霉素 脂质体 包封率 |
| 文章编号： | 1000-5501(2008)02-0165-03 |
| 修稿时间： | 2007年11月23 |
Preparation and quality control of epirubicin hydrochloride liposomes |
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| WU Yan,WU Cheng,KANG Yan-Min,MEI Xing-Guo.Preparation and quality control of epirubicin hydrochloride liposomes[J].Bulletin of the Academy of Military Medical Sciences,2008,32(2):165-167. | |
| Authors: | WU Yan WU Cheng KANG Yan-Min MEI Xing-Guo |
| Institution: | WU Yan1,WU Cheng2,KANG Yan-Min3,MEI Xing-Guo1 (1.Institute of Pharmacology , Toxicology,Academy of Military Medical Sciences,Beijing 100850,China,2.The Second Artillery General Hospital,Beijing 100088,3.College of Pharmacy,Yanbian University,Yanji,Jilin 133000,China) |
| Abstract: | Objectives: To prepare epirubicin hydrochloride liposomes(Epi-lip),establish an HPLC method for the determination of EPI and to optimize the method for determination of entrapment efficiency(EE%).Methods:Epi-lip was prepared by thin film-pH gradient method.Sephadex-G50 chromatography,the ultrafiltration method and dialysis method were used to determine EE% of liposomes.Results:The EPI-lip had a small particle size and narrow size distribution.The EE% of liposomes was 96.1%,98.4% and 92.9% determined by Seph... |
| Keywords: | pH gradient method epirubicin hydrochloride liposome entrapment efficiency |
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