| HPLC法测定草酸阿德福韦酯片溶出度 |
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| 引用本文： | 王喜军,杨蕾.HPLC法测定草酸阿德福韦酯片溶出度[J].中外医疗,2013,32(17):18-19. |
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| 作者姓名： | 王喜军 杨蕾 |
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| 作者单位： | 王喜军 (哈药集团制药总厂,黑龙江哈尔滨,150046)； 杨蕾 (哈药集团制药总厂,黑龙江哈尔滨,150046)； |
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| 摘 要： | 目的采用高效液相色谱法测定草酸阿德福韦酯片的溶出度。方法按照中国药典2010年版二部溶出度测定法中的第二法,以0.1mol/L HCl600mL为溶出介质,转速为50rpm,45min时采样,以高效液相色谱法测定。色谱柱：DiamonsilC18（150mm×4.6mm,5μm）;流动相：甲醇-水-冰醋酸=50：50：0.1;流速：1.0mL/min;检测波长：258nm,以外标法测定草酸阿德福韦酯片的溶出度。结果线性范围为9.42～22.62μg/mL。平均回收率为100.2%。（RSD为0.50%,n=9）,溶出度符合规定。结论该方法简便、易行,结果准确。
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| 关 键 词： | 草酸阿德福韦酯片 溶出度 高效液相色谱法 |
Deter mination of dissolution of Adefovir Dipivoxil Oxalic Acid tablets by HPLC |
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| WANG Xijun,YANG Lei.Deter mination of dissolution of Adefovir Dipivoxil Oxalic Acid tablets by HPLC[J].China Foreign Medical Treatment,2013,32(17):18-19. |
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| Authors: | WANG Xijun YANG Lei |
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| Institution: | Harbin Pharmaceutical Group Co.Ltd.General Pharm.Factory of Harbin City,Heilongjiang Province,Harbin 150046,China |
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| Abstract: | Objective The research establish HPLC methods for dissolution of Adefovir Dipivoxil Oxalic Acid tablets.M ethods Perform the test according to apparatus II,the medium is 0.01mol/L hydrochloric acid,600ml,50rpm.The dissolution time is 45min.Use HPLC method for the determination.Diamonsil C18 column（150mm ×4.6mm,5μm） is used with methanol,water and glacial acetic acid（50：50：0.1） as the mobile phase,with flow rate of 1.0mL/min and the detection wavelength of 258nm.Results The linear range of Adefovir Dipivoxil is 9.42～22.62 μg/mL,the average recovery is 100.2% and the RSD is 0.50%.C onclusion The method is convenient,sensitive and accurate for the determination of the drug. |
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| Keywords: | Adefovir Dipivoxil Oxalic Acid tablets Dissolution HPLC method |
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