孕期酒精暴露对子代大鼠学习记忆及海马N-甲基-D-天冬氨酸受体2B亚基表达的影响

目的 研究孕期酒精暴露对子代大鼠学习记忆及海马N-甲基-D-天冬氨酸(NMDA)受体2B亚基(NR2B)表达的影响.方法 按照随机数字表法,将雌性SD大鼠随机分为正常对照组、饮酒对照组和孕期酒精暴露组,每组各8只；饮酒法建立大鼠孕期酒精暴露模型,子代成年后,采用Y-型迷宫测试子鼠学习记忆成绩；采用聚合酶链反应分析子鼠海马组织NR2B mRNA的表达；采用免疫荧光法检测子鼠海马区NR2B蛋白表达.结果 (1)各组子鼠成年后学习记忆成绩的差异有统计学意义(F=4.566,P＜0.05),孕期酒精暴露组子鼠学习记忆成绩(43.00±15.33)次]比正常对照组 (25.13±12.35)次]和饮酒对照组(26.12±11.95)次]明显下降(P均＜0.05)；(2)各组子鼠成年后海马组织中NR2B mRNA表达差异有统计学意义(F=29.795,P＜0.01),孕期酒精暴露组子鼠海马区NR2B mRNA表达(0.97±0.14)较正常对照组(0.52±0.10)和饮酒对照组(0.62±0.12)明显上升(P均＜0.01)；孕期酒精暴露组子鼠海马区NR2B蛋白表达明显增加.结论 孕期酒精暴露对子代大鼠的神经损伤可能与NMDA受体亚基NR2B蛋白表达的上调有关.

Effect of alcohol exposure during pregnancy on learning and memory and expression of N-methyl-Daspartate receptor 2B subunit in the hippocampus of infant rats

Objective To observe the effect of alcohol exposure during pregnancy on learning and memory and expression of N-methyl-D-aspartate receptor 2B subunit(NR2B) in the hippocampus of infant rats.Methods Female adult SD rats were randomly divided into control group,drinking group and alcohol exposure during pregnancy group.The animal models of alcohol exposure during pregnancy were made by given drinking water with successively increased concentration of ethanol before pregnancy,and the learning and memory were evaluated by Y-maze in adult offspring. The expression of NR2B mRNA in the hippocampus were evaluated with RT-PCR,and NR2B protein expression by immunofluorescence.Results Y-maze test showed that the learning and memory of each groups were different( F =4.566,P ＜ 0.05 ),the alcohol exposure during pregnancy group (43.00 ± 15.33) was significantly decreased compared with that of control group (25.13 ± 12.35 ) and drinking group ( 26.12 ± 11.95 ) ( P ＜ 0.05 ).RT-PCR results showed that the expression of NR2B mRNA of each groups were different ( F =29.795,P ＜ 0.01 ),the alcohol exposure during pregnancy group (0.97 ± 0.14 ) was significantly increased compared to control group (0.52 ±0.10) and drinking group ( 0.62 ± 0.12 ) ( P ＜ 0.01 ).Immunofluorescence showed that the expression of NR2B protein of alcohol exposure during pregnancy group was significantly increased.Conclusion The damage effect of alcohol exposure during pregnancy on infant rats nerve system may interrelate with up-regulation of N-methyl-D-aspartate receptor subunit NR2B in the hippocampus.