牛磺酸对长期大强度运动训练后大鼠自由基代谢、膜流动性及钙转运的影响

70只雄性Wistar大鼠（80-100g），随机分为3组：对照组（G1）、游泳训练组（G2）和游泳训练＋牛磺酸组（G3）。牛磺酸补充方式为每日灌服1次（500mg／Kg）。递增负荷训练6周，观察牛磺酸对长期大强度运动训练后大鼠自由基代谢、膜流动性及钙转运的影响。结果显示：运动后即刻，G3组血NH3、BLA、BUN明显低于G2组（P＜0．05）；运动后24小时，G3组血NH3、BLA与对照组比较已无显著性差异（P＞0．05），而G3组BUN明显低于G2组（P＜0．05）。运动后即刻及24小时，G3组RBC、血浆及心肌线粒体MDA含量明显低于G2组（P＜0．05）；G3组RBC及心肌线粒体GPX活力明显高于G1和G2组（P＜0．05）；G3组心肌线粒体膜荧光偏振度P明显低于G2组（P＜0．05）；G3组心肌线粒体Ca2+浓度明显低于G2组（P＜0．05）；G3组SRCa2+－Atpase活性和摄钙率明显高于G2组（P＜0．05）。以上结果表明，牛磺酸的抗自由基损伤，稳定生物膜和调节钙转运作用可能是其对抗运动性疲劳的重要机制。

Effects of Taurine on Free Radicals Metabolism ,Membrane Fluidity and Calcium Transfer after Chronic Exercise in Rats

To observe the effects of taurine on free radicals metabolism, membrane fluidity and calcium transfer ther chronic exercise, 70 maleWistar rats wererandomly divided into three groups as follows: control group (G1 ), exercise group (G2), exercise taurine group (G3).Taurine-treated animals (G3) received forced oral administration of 500 mg/kg taurine per day for sir weeks by means of a cannula inserted into the esophagus. Other animals received the same amount of water each day as above. Training intensity of the rats of two excise groups (G2and G3) were gradually increased. At the end of the sixth week, all rats of each group were killed and different samples were collected. Theresults were as follows: 1) Blood NH3, BLA and BUN were increased after exercise in G2, but not in G3; 2) MDA contents of RBC, Plasma and mitochondria (Mit) were all increased immediately and 24h after exercise, whereas taurine supplements reduced this increase; 3)GPX activities of RBC and Mit were incrend in taurine-supplemented rats ther exercise; 4) The fluidity of Mit were decreased after exercise, while taurine reduced this decrease; 5) Mit-Ca2 contents was increased immediately and 24h after exercise, but taurine preventedfrom the increase. 6. SR Ca-ATPase activity and the rate of Ca2 uptake were decreased immediately ther exercise, tauine reduced the decrease and promoted restoration after exercise. The results suggested that the effects of taurine on free radicals metabolism, membrane flnidityand calcium transfer could be important in the mechanisms of antifatigue.