反复自然流产患者外周血CD4~+CD25~+调节性T细胞的检测

目的:探讨CD4+CD25+调节性T细胞(Tr)在反复自然流产发病机制中的作用。方法:应用流式细胞术检测29例原因不明的反复自然流产妇女(URSA组)和20例正常妊娠妇女(正常对照组)外周血中CD4+CD25+Tr的比例。结果:URSA组外周血中CD4+CD25brightTr百分率(1.98±0.96)%]显著低于正常对照组(3.21±1.25)%,P<0.05],而2组间CD4+CD25+总百分率、CD4+CD25dim细胞百分率以及CD4+CD25bright/CD4+比值均无显著性差异(P均>0.05)。结论:URSA的发生可能与患者CD4+CD25brightTr比例下降有关,CD4+CD25brightTr在母胎免疫耐受中发挥重要作用。

Evaluation of CD4~+CD25~+ Regulatory T Cells in the Peripheral Blood of Recurrent Spontaneous Abortion Patients

Objective ： To explore the role of CD4^＋CD25^＋ regulatory T cells （ CD4^＋CD25^＋ Tr） in the pathogenesis of recurrent spontaneous abortion. Methods ： Peripheral blood samples were collected from 29 women with unexplainable recurrent spontaneous abortion （ the URSA group） and another 20 with normal pregnancy （ the control group）. The percentage ofCD4^＋CD25^＋ Tr in the peripheral blood was measured by flow cytometry. Results ： The rate of CD4^＋CD25^bright Tr in the URSA patients （ 1.98±0.96 ] % ） was significantly lower than that in the control group （ 3.21 _＋ 1.25 ] %, P 〈 0.05 ）, while the percentages of CD4^＋CD25^＋ and CD4 ^＋CD25^dim and the ratio of CD4^＋CD25^bright/ CD4^＋ were not significantly different between the two groups （P 〉 0.05）. Conclusion： URSA might be associated with the decreased percentage of CD4^＋CD25^bright Tr, which plays an important role in fetomatemal immunologic tolerance.