荧光金逆行标记乳鼠视网膜神经节细胞的实验研究

目的建立荧光金逆行标记乳鼠视网膜神经节细胞的方法。方法选取出生1 d的SD大鼠乳鼠,腹腔注射水合氯醛麻醉后,剪开头部皮肤,向双侧上丘各注射4%荧光金注射液1μl,缝合皮肤,4 d后处死,提取视网膜,胰酶消化后培养,显微镜下观察被标记的视网膜神经节细胞。结果混合培养的视网膜细胞中,可见被荧光金标记的视网膜神经节细胞,大约占细胞总数的0.34%,视网膜神经节细胞可存活3周。结论荧光金逆行标记乳鼠视网膜神经节细胞是一种可行的方法,可用于视网膜神经节细胞的鉴定。

Research on retinal ganglion cells of neonatal rats labeled retrogradely with fluorogold

Objective To establish a method to label the retinal ganglion cells of neonatal rats with fluorogold.Methods One-day neonatal Sprague-Dawley rats were anesthetized by intraperitoneal injection with chloral hydrate,and then 1 μl of 4% fluorogold was microinjected bilaterally into the superior colliculi of rats.Four days later,primary mixed retinal cell cultures were established from retinas of five-day neonatal rats.Results Under fluorescence microscope,retinal ganglion cells in mixed cultured retinal cells were successfully labeled with fluorogold,which accounted for 0.34% of total retinal cells.In addition,retinal ganglion cells survived for three weeks in vitro.Conclusion Retinal ganglion cells of neonatal rats labeled by fluorogold is a feasible approach,and could contribute to the identification of retinal ganglion cells in primary mixed retinal cell cultures.