氧化苦参碱对癫痫大鼠学习记忆功能及海马神经元的影响

目的观察氧化苦参碱(oxymatrine,OXY)对青霉素(penicillin,PEN)致痫大鼠学习记忆功能和海马神经元形态的影响。方法成年SD大鼠48只随机分为生理盐水对照组(normal saline,NS)、癫痫模型组(epilepsy,EP)、氧化苦参碱组(OXY)和苯巴比妥钠阳性对照组(PBS),腹腔注射青霉素诱导大鼠癫痫发作,观察大鼠行为学改变,Morris水迷宫检测大鼠空间学习和记忆,观察大鼠海马区神经元细胞形态学改变。结果通过Morris水迷宫视频跟踪系统观察各组大鼠在A区和C区停留的时间和在A区和C区游泳路径的长度,PBS组和OXY组在A区的停留时间和在A区的游泳路径比EP组明显增长(P<0.05),对大鼠空间记忆能力有所改善。NS组神经细胞结构正常;EP组可见神经细胞肿胀、细胞器的损伤等改变;PBS组变性坏死程度较轻,大部分神经细胞胞膜都较完整;OXY组神经元细胞肿胀明显减轻,细胞器等损伤能够得到改善。结论 OXY可以对癫痫大鼠在空间学习和记忆方面起到保护作用。

Effect of oxymatrine on learning and memory and hippocampus neuron in epileptic rats

Objective To explore the effects of oxymatrine on learning and memory and the hippocampus neurons in epileptic rats induced by penicillin.Methods Forty-eight SD rats were randomly divided into normal saline(NS)group,epilepsy(EP)group, oxymatraine(OXY)group and phenobarbital sodium(PBS)group.Penicillin was injected intraperitoneally in rats to establish the epilepsy model.Then behavioral changes were observed.The stay time of rats and the swimming length of rats in regions A and C were observed through Morris water maze video tracking system.The spatial learning and memory was tested by Morris water maze(MWM). And the changes of hippocampus neuron in rats were observed.Results The stay time of rats and the swimming length of rats in region A in PBS group and OXY group were longer than those in EP group(P<0.05).The abilities of learning and memory in OXY group were significantly improved.After epilepsy induced by penicillin,the neuron tumefaction and organelle damnification were found. But after using OXY,the neuron tumefaction and organelle damnification were alleviated.Conclusion OXY could protect the spatial learning and memory in epileptic rats.