| 人卵体外受精、胚胎培养的初步报告 |
| |
| 引用本文： | 卢光绣,范立青,李胜,廖志坚,卢惠霖,朱敬璋,陈涤霞,徐立礼,张志胜.人卵体外受精、胚胎培养的初步报告[J].中南大学学报(医学版),1987(4). |
| |
| 作者姓名： | 卢光绣 范立青 李胜 廖志坚 卢惠霖 朱敬璋 陈涤霞 徐立礼 张志胜 |
| |
| 作者单位： | 湖南医学院生殖工程研究室
(卢光绣，范立青，李胜，廖志坚，卢惠霖)，湖南医学院第一附属医院妇产科
(朱敬璋，陈涤霞)，湖南医学院第二附属医院妇产科
(徐立礼)，湖南医学院第二附属医院妇产科(张志胜) |
| |
| 摘 要： | 分别从15个用药物诱导周期和21个自然卵泡发育周期的妇女抽吸了175个卵泡的卵泡液,获得不同成熟度的卵母细胞74个,孵育后以丈夫或供者的精子体外授情,共得受精卵42个,受精率为56.7%,34个发生卵裂,发育至2-细胞至桑椹胚阶段,体外受精卵裂率为45.9%。卵母细胞以成熟度分类,中间状态卵的体外受精率(76.2%)及卵裂率(64.3%)为最高。
|
| 关 键 词： | 卵母细胞 体外受精 排卵诱导 生育药 女 激素性 分裂期 卵 |
A PRELIMINARY REPORT OF IN VITRO FERTILIZATION AND ULTURE OF HUMAN OOCYTES |
| |
| Lu Guangxiu,Fan Liqing,Huang Shanbao,Li Sheng,Liao Zhijian,Lu Huilin Hunan Reproductive Engineering Laboratory,Hunan Medical CollegeZhu Jingzhang,Chen Dixia,Xu Lili,Zhang Zhisheng.A PRELIMINARY REPORT OF IN VITRO FERTILIZATION AND ULTURE OF HUMAN OOCYTES[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1987(4). |
| |
| Authors: | Lu Guangxiu Fan Liqing Huang Shanbao Li Sheng Liao Zhijian Lu Huilin Hunan Reproductive Engineering Laboratory Hunan Medical CollegeZhu Jingzhang Chen Dixia Xu Lili Zhang Zhisheng |
| |
| Institution: | Lu Guangxiu,Fan Liqing,Huang Shanbao,Li Sheng,Liao Zhijian,Lu Huilin Hunan Reproductive Engineering Laboratory,Hunan Medical CollegeZhu Jingzhang,Chen Dixia,Xu Lili,Zhang Zhisheng Department of Grnecology and Obstetrics,The First and The Second Affiliated Hospital,Hunan Medical College |
| |
| Abstract: | Total 175 follicles were individually aspirated from 15 cycles stimulated with clomiphene,hMG and hCG and from 21 natural(unstimulated)cycles.74 oocytes of various mature degree were recovered.After a 6-8 hours preincubation in the modified Ham's F-10 media,the eggs were inseminated with sperm of husband or donor washed.42 eggs were fertilized in vitro(56.7%),of which 34 fertilized oocytes cleavage in a 2-cell to morula-stage embryos(rate of cleavage was 45.9%).The oocytes were identified according to the morphology of oocyte-corona-cumulus complex which was described by Neri Laufer et al.The result indicated that intermediate oocytes had highest rate of fertilization(76.2%)and cleavage (79.4%). |
| |
| Keywords: | Oocyte Fertilization in vitro Ovulation induction Fertility agents female hormonal Cleavage stage ovum |
| 本文献已被 CNKI 等数据库收录！ |
|
