白细胞介素-1基因多态性与侵袭性牙周炎的关系

目的：研究中国人群中白细胞介素-1（IL-1）基因多态性与侵袭性牙周炎（AgP）之间的关系。方法：提取122例AgP患者和95例健康对照者外周静脉血基因组DNA，采用聚合酶链反应（PCR）分析IL-1A＋4845、IL-1B＋3954位点的单核苷酸多态性（SNP）及IL-1RN第二内含子中的可变数目重复序列（VNTR）多态性，采用多因素Logistic回归模型，进行两组之间基因型、等位基因分布差异的比较。结果：在IL-1A＋4845位点，AgP组男性患者A2^＋基因型的频率较男性健康组显著升高（P〈0．05=0．039），等位基因A2的携带率也显著升高（P〈0．05=0．049）；未发现IL-1B＋3954位点和IL-1RN VNTR的多态性与AgP关联；未发现IL-1各复合基因型与AgP存在明显关联性。结论：IL-1A＋4845位点的SNP可能与中国人群中男性个体的AgP易感性有关。

Interleukin-1 polymorphisms in patients with aggressive periodontitis

PURPOSE: To study whether interleukin- 1 (IL- 1) genotypes and/or alleles were associated with aggressive periodontitis (AgP). METHODS: Anti- coagulated peripheral blood samples were obtained from 122 AgP patients and 95 healthy controls. Genomic DNA was extracted from each sample. Single nucleotide polymorphisms (SNPs) at IL- 1A +4845 and IL- 1B +3954 were analyzed by standard polymerase chain reaction- restriction fragment length polymorphism (PCR- RFLP) assay. The polymorphism of a variable number tandem repeat (VNTR) in intron 2 of IL- 1RN was detected by PCR amplification and fragment size analysis. The genotypes and/or alleles distribution differences between two groups were analyzed by logistic regression analysis. RESULTS: The frequencies of A2+ genotype and allele 2 at IL- 1A +4845 were significantly increased in male AgP patients compared with male controls (genotype: 18.0% vs 4.3%, adjusted OR=5.58, 95% CI=1.09- 28.68,P=0.039;allele: 9.0% vs 2.2%, adjusted OR=4.97, 95% CI=1.01- 24.50, P=0.049). CONCLUSIONS: The IL- 1A +4845 polymorphism may be associated with AgP susceptibility in Chinese males. Supported by National Natural Science Foundation of China (Grant No.30271411).