Quantitative Analysis of Hydroxyurea and Urea by Proton Nuclear Magnetic Resonance (NMR) Spectroscopy |
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| Authors: | Main Karen B. Medwick Thomas Bailey Leonard C. Shinkai John H. |
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| Affiliation: | (1) Rutgers University, College of Pharmacy, Box 789, Piscataway, New Jersey, 08854 |
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| Abstract: | Quantitative 1H-nuclear magnetic resonance (NMR) procedures are described for measuring hydroxyurea and urea. Dimethylsulfoxide-d6 is used as the solvent for both assays; the internal standards employed are urea and p-dichlorobenzene for hydroxyurea and urea, respectively. The analysis for hydroxyurea is based on the comparison of the area of the – NH2 peak of hydroxyurea with the area of the urea – NH2 peak area. The analysis of urea is based on the comparison of the – NH2 peak of urea with the area of the p-dichlorobenzene singlet. The 1H-NMR assays yield results that are precise and agree well with results of the more cumbersome and less specific USP procedures. |
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| Keywords: | hydroxyurea urea quantitative 1H-NMR |
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