PDGF在骨髓问充质干细胞向胶质瘤趋化迁移过程中的作用

目的 观察血小板衍生生长因子(PDGF)和胶质瘤细胞条件培养基对人骨髓间充质干细胞(hMSCs)体外迁移能力的影响,探讨PDGF在hMSCs向胶质瘤趋化迁移过程中的作用. 方法 全骨髓贴壁细胞法分离培养hMSCs,应用RT-PCR检测hMSCs中PDGF受体α(PDGFR-α)和β(PDGFR-β)的表达;利用聚碳酸酯膜小室建立体外趋化迁移模型,观察5、50、125 ng/mL PDGF和胶质瘤细胞条件培养基对hMSCs的体外趋化作用以及加入PDGF抗体后对hMSCs趋化迁移的影响. 结果 RT-PCR检测到hMSCs表达PDGFR-α和PDGFR-β;PDGF可以浓度依赖性促进hMSCs的趋化迁移.胶质瘤细胞条件培养基也可趋化hMSCs的迁移.与空白对照组相比迁移细胞数差异均有统计学意义(JP<0.05);PDGF抗体可消除50 ng/mL PDGF、减弱胶质瘤细胞条件培养基对hMSCs趋化迁移的作用. 结论 PDGF促进hMSCs的趋化迁移.在hMSCs向胶质瘤选择性迁移过程中发挥"趋化因子"样作用.

Role of platelet-derived growth factor in directional migration of human meseuchymal stem cells toward gliomas

Objective To investigate the effect ofplatelet-derived growth factor (PDGF) and conditional medium of U87 glioma cells on the migration ability of human mesenchymal stem cells (hMSCs) to understand the possible role of PDGF in the directional migration of hMSCs toward gliomas. Methods hMSCs were isolated from the whole bone marrow by adherent culture, and the expression of PDGF receptor (PDGFR-α, β) in the cells was examined by RT-PCR. In vitro migration assay was performed using transwell inserts to observe the effect of PDGF (0, 5, 50, and 125 ng/mL) and the conditional medium on the directional migration ability ofhMSCs. The changes in the migration ability of hMSCs in response to addition of anti-PDGF antibody in the conditional medium were investigated. Results RT-PCR detected the expression of PDGFR- αand PDGFR-β mRNA in the isolated hMSCs. In the cell migration assay, both PDGF and the conditional medium induced directional migration of hMSCs (P<0.05), which was significantly suppressed by anti-PDGF antibody P<0.05). Conclusion With chemokine-like activities, PDGF concentration-dependently enhances the directional migration of hMSCs toward gliomas in vitro.