鲍曼不动杆菌环丙沙星耐药性接合转移的实验研究

目的:探讨质粒转移与鲍曼不动杆菌环丙沙星(CIP)耐药性的关系。方法:将鲍曼不动杆菌CIP耐药菌株(供体菌)与E.coli(C600SMRLac-)(受体菌)进行接合试验,采用琼脂稀释法测定供体菌及接合子的CIPMIC值。结果:16株CIP耐药性鲍曼不动杆菌,其中5株CIP耐药株与受体菌成功发生了接合,CIP耐药性接合转移的发生率为31.25%(5/16)。接合子的CIPMIC值比受体菌增加了30～500倍,其中1株接合子对CIP呈现低水平耐药。结论:部分鲍曼不动杆菌的CIP耐药性与质粒有关,且能够通过接合方式在细菌之间发生转移。

Experimental Study on Conjugation Transfer of Ciprofloxacin Resistance in Acinetobacter baumanii

Objective： To investigate the relationship between plasmid transfer and ciprofloxacin resistance in A.baumanii. Methods： The conjugation experiments were carried out with ciprofloxacin-resistant A.baumanii as donor strains and E.coli （C600 SMR Lac ） as the recipient, and MICs for the donor, recipient, and transconjugant strains were measured by agar dilution. Results： Five strains of ciprofloxacin-resistant A. baumanii were conjugated with the recipient, and the incidence rate of conjugation transfer of ciprofloxacin resistance was 31.25%（5/16）. Ciprofloxacin MICs for the transconjugant strains increased by 30 to 500 times than that of the recipient sWain, and one strain of the transconjugant was low-level ciprofloxacin resistance. Conclusion： In some isolates of A. baumanii, the ciprofioxacin resistance is realted to the plasmid, and can be transferred to other bacteria by conjugation.