|
|||||
|
|
| Whole CanA Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism | |
| 作者姓名： | 叶嗣颖 敖杰男 彭颖 岳海峰 廖芳 胡国平 徐杨 张正茂 |
| 作者单位： | [1]DepartmentofPathogenirOrganisms,SchoolofBasicMedicalSciences,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030 [2]MedicalCollegeofJinanUniversity,Guangzhou510 |
| 摘 要： | To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP),nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate the RFLP fingerprinting.Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained.It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile. |
| 关 键 词： | 幽门螺旋杆菌 核心抗原A PCR RFLP |
| 收稿时间： | 1 March 2002 |
Whole CagA gene amplification ofhelicobacter pylori and its fingerprinting by restriction fragment length polymorphism |
|
| Siying Ye M. D., Ph. D.,Jienan Ao,Ying Peng,Haifeng Yue,Fang Liao,Guoping Hu,Yang Xu,Zhengmao Zhang.Whole CanA Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2002,22(4):276-278. | |
| Authors: | Siying Ye M D Ph D Jienan Ao Ying Peng Haifeng Yue Fang Liao Guoping Hu Yang Xu Zhengmao Zhang |
| Institution: | 1. Department of Pathogenic Organismns, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan,430030 2. Medical College of Jinan University, Guangzhou,510000 3. Center of Cellular and Molecular Biology, Wenzhou Medical College, Wenzhou,325000 4. Department of Microbiology ＆ Infectious Diseases, University of Calgary Faculty of Medicine, Calgary, Canada AB T2N4N1 |
| Abstract: | Summary To set up a method of amplification for the whole CagA gene ofHelicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile. This project was supported by a grant from the Ministry of Public Health (Serial No.: 98-1-123). |
| Keywords: | Helicobacter pylori cagA PCR RFLP |
| 本文献已被 维普 万方数据 SpringerLink 等数据库收录！ | |