黄芪对大鼠心肌缺血再灌注损伤的防护作用

麻醉开胸大鼠结扎冠脉左室支３０ｍｉｎ后再灌注６０ｍｉｎ造成大鼠心肌缺血再灌注损伤，在再灌注的同时恒速ｉｖ黄芪（８３０ｍｇ／ｋｇ）对上述心肌损伤有明显的防护作用，表现为明显降低再灌注性心律失常室速（ＶＴ）及室颤（ＶＦ）的发生率，延迟其出现时间并缩短其持续时间，促进缺血型ＳＴ段的恢复。减少心肌细胞内Ｃａ２＋的蓄积及细胞内磷酸肌酸激酶同功酶（ＣＫ－ＭＢ）的漏出。明显降低心肌组织中ＭＤＡ含量并显著提高ＳＯＤ和ＧＳＨ－Ｐｘ的活性。结果提示黄芪防护心肌缺血再灌注损伤的作用与其提高心肌的抗氧化能力、清除氧自由基、减轻细胞膜脂质过氧化状态和维持细胞膜的完整性相关。

Protective Effect of Astragalus Membranaceus on Myocardial Ischemia Reperfusion Injury in Rats

The protective effects of Astragalus membranaceus (Am) on myocardial ischemia reperfusion injury were investigated in a myocardial ischemia reperfusion model induced by 30min coronary occlusion and 60min reperfusion in open chest anesthetized rats. It was found that Am effectively antagonized the reperfusion injury. 1) It reduced the incidence of reperfusion ventricular arrhythmias (VT and VF), retarded the appearing time, shortened the duration and lowered the elevated ST segment. 2) It decreased the Ca 2 accumulation and creatine kinase-MB (CK-MB) release in reperfused myocardium. 3) It decreased malondialdehyde (MDA) content and increased the superoxide dimutase (SOD) and glutathione peroxidase (GSH-Px) activities. the results suggested that the protected effects of AM on the myocardial ischemia reperfusion injury may be related to its action of scaverging the oxygen free radicals, protecting the antioxygen free radical enzymes and inhibiting the lipid peroxidation in the myocardium.