尼莫地平预处理和梗塞后治疗对大鼠脑缺血损伤保护作用的比较研究

目的:探讨尼莫地平(Nimodipine,NIM)预处理和梗塞后给药对脑缺血区神经元保护作用的差异。方法:采用线栓法建立大鼠脑缺血模型;尼莫地平给药方式为单预处理、单梗塞后给药或预处理+梗塞后治疗;红四氮唑(TTC) 染色测定脑梗塞体积百分比;逆转录聚合酶链式反应(RT-PCR)法测定动物海马组织中Caspase-3 mRNA和Bcl-2 mR NA的相对表达量。结果:尼莫地平预处理+梗塞后治疗组、单预处理组及单治疗组的脑梗塞体积百分比均显著小于对照组,而前三组相比,预处理+梗塞后治疗组的脑梗塞体积百分比小于单预处理组及单治疗组,但单预处理组及单治疗组间无明显差异。Caspase-3 mRNA表达量为对照组最高,单治疗组次之,单预处理组又次,预处理+梗塞后治疗组最低;Bcl-2mRNA在各组的表达量与Caspase-3 mRNA相反。结论:尼莫地平预处理+梗塞后治疗的方式对缺血神经元的保护作用最强,单预处理次之,而单梗塞后治疗的保护作用最弱。

A Study of the Protective Role of Pre-treated or Treated after Ischemia Onset with Nimodipine on Rats Cerebral Ischemia Model

Objective: To study the differences of the protective role between pre-treated and treated after ischemia onset with Nimodipine on the neurons in cerebral ischemia areas. Methods:The cerebral ischemic model of rat was made by occluding left middle cerebral artery according to Nagasawy and Zea Longa improvement method. The rats were pre-treated, treaded after ischemia onset or pre-treated plus treaded after ischemia with Nimodipine. The percentage of the infarction volume was calculated by red tetrazoline (ITC) pigmentation. The level of Caspase-3 and Bcl-2 mRNA were measured by RT-PCR method. Results: The percentage of the cerebral infarction volume of pre-treated, treated after ischemia and pre-treated plus treaded after ischemia with Nimodipine was significant lower than that of control, and in the above three groups, the infarction percentage of the last group was lower than the anterior two. However, there was no significant difference between the percentage of pre-treated group and the one of treated after ischemia. The level of Caspase-3 mRNA was the highest in control group, and the expression was lower in treated after ischemia group, pre-treated group and pre-treated plus treated after ischemia group in turn. The level of Bcl-2 mRNA was reversed in the four groups. Conclusion: There was stronger protective effects of pre-treated plus treated after ischemia with Nimodipine than pre-treated or treated after ischemia.