大鼠睾丸间质祖细胞移植的实验研究

目的探讨去势大鼠模型进行睾丸间质祖细胞（progenitor leydig cell，PLC）移植的可行性。方法制作去势大鼠模型，将经过体外培养、鉴定的PLC移植入肾包膜内，采用RT—PCR及磁分离均相酶联免疫（磁酶免）睾酮定量测定法等技术方法观察其移植后生长分化情况。结果移植后受体血清睾酮浓度明显高于同期去势对照组（P〈0．01）。移植12周后实验组精囊的长度和相对重量明显高于对照组（P〈0．01）。RT—PCR定量检测促黄体生成素受体（LHR）mRNA，对照组阴性表达，实验组第4周LHRmRNA表达较第8周及第12周低（P〈0．01），第8周组与第12周组LHR表达差异无统计学意义（P〉0．05）。结论移植的PLC在去蛰大鼠的肾包膜内可以存活．且逐渐生长分化成熟。可在一定程度弥补生殖功能不足。

Experimental study on the rat progenitor leydig cell transplantation

Objective To investigate the feasibility of transplanting the progenitor Leydig cell（PLC） into the emasculated rat model. Methods The model of emasculated rat was established. After cultured in vitro, the rat progenitor Leydig cell was transplanted into the kidney capsule, the status of the proliferation and differentiation was observed by RT-PCR and magnetic enzyme immunoassay（testosterone quantitative determination）. Results The blood serum testrone concentration of the host was significantly higher than that of control group（ P 〈0.01 ）. The length and the relative weight of the rats＇ seminal vesicle in experimental group were significantly higher than those of control group after 12 weeks（ P 〈 0.01 ）. LHRmRNA was quantitatively measured by RT-PCR,and the control group was negative, however, the expression of LHRmRNA in the experimental group on the 4th week was significantly lower than that on the 8th week and the 12th week（ P 〈0.01 ）. There was no significant difference in the expression of LHRmRNA on the 8th week and the 12th weekbetween experimental group and control group（ P 〉 0.05 ）. Conclusion The transplanted progenitor Leydig cells can proliferate inside the emasculated rats＇ kidney capsule, and can improve the breeding ability of the host at some extent.