人乳头瘤病毒分型与宫颈病变关系的研究

目的探讨不同砸别人乳头瘤病毒（Human papilloma virus，HPV）感染与宫颈疾病发生发展的关系。方法应用核酸分子快速杂交基因分型技术，对375例宫颈病变患者（宫颈炎182例、CINI81例、CINⅡ68例、CINⅢ69例、宫颈癌15例）进行HPV感染基因型分型测定，同时进行宫颈细胞学检查。结果375例标本中HPV阳性检出率为35．2％，HPV在各类宫颈病变组的阳性枪出率分别为宫颈炎18．6％，CINI32．1％，CINⅡ57．3％，CINⅢ68．9％，宫颈癌86．7％，宫颈炎组和宫颈癌病变组HPV之间结果差异有统计学意义（x^2=105．0，P〈0．05）。HPV多重感染率为32．6％，其中最常见HPV感染亚型为HPV16。结论宫颈病变严重程度与感染HPV亚型的致病能力密切相关。HPV核酸分型检测对宫颈疾病诊断和治疗具有重要意义。

The relationship between human papolluma virus subtypes and cervical lesion

Objective To investigate the relationship between human papilloma virus DNA（ HPV- DNA） type and the cervical lesion. Methods 375 cases with cervical lesion （ inclued 182 chronic cervicitis,81 CIN I , 68 CIN]] , 69 CINm and 15 cervical cancer） were determined as HPV diagnosed by DNA flow-through hybridization genotyping technique. Cervical cytological examination were also performed. Resuits HPV infection rate of the 375 samples was 35.2%. The infection rate of HPV in chronic cervicitis, CINI , CINⅡ, CINⅢ and cervical cancer were 18.6 %, 32.1%, 57.3 %, 68.9 % and 86.7 %, respectively. There was statistics difference among these cases（ X^2 = 105.0, P 〈 0. 05）. Conclusion There is relationship between the severity of cervical lesions and HPV genetype. HPV genotyping is essential for early diagnosis and treatment.